VIP Polyclonal antibody

VIP Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF

Conjugate

Unconjugated

Publications

2

Cat no : 16233-1-AP

Synonyms

PHM27, vasoactive intestinal peptide, VIP, VIP peptides

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Validation Data Gallery

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  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16233-1-AP (VIP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16233-1-AP (VIP Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16233-1-AP (VIP Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16233-1-AP (VIP Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of mouse brain using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of COLO 320 using 16233-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 16233-1-AP (VIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse colon using 16233-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using VIP antibody (16233-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Calretinin antibody (66496-1-Ig, Clone: 2D7A9, red).

• 

  IF Staining of mouse colon using 16233-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using VIP antibody (16233-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Calretinin antibody (66496-1-Ig, Clone: 2D7A9, red).

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Tested Applications

• Positive IHC detected in: human colon tissue, human small intestine tissue, mouse brain tissue, rat small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: COLO 320 cells, mouse colon tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 2 publications below

Product Information

16233-1-AP targets VIP in IHC, IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VIP fusion protein Ag8933
• Full Name: vasoactive intestinal peptide
• Calculated molecular weight: 169 aa, 19 kDa
• GenBank accession number: BC009794
• Gene symbol: VIP
• Gene ID (NCBI): 7432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Pharm Biol; Modified BuShenYiQi formula alleviates experimental allergic asthma in mice by negative regulation of type 2 innate lymphoid cells and CD4 + type 9 helper T cells and the VIP-VPAC2 signalling pathway; Authors - Muhua Huang
mouse	IF	Cell Rep; PMID: 31509750; Authors - Jing Xue