CoraLite® Plus 488-conjugated VIP Polyclonal antibody

VIP Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16233

Synonyms

PHM27, vasoactive intestinal peptide, VIP, VIP peptides

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Validation Data Gallery

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  IF Staining of mouse colon using CL488-16233

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 488 VIP antibody (CL488-16233) at dilution of 1:200.

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  IF Staining of mouse colon using CL488-16233

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 488 VIP antibody (CL488-16233) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse colon tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16233 targets VIP in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VIP fusion protein Ag8933
• Full Name: vasoactive intestinal peptide
• Calculated Molecular Weight: 169 aa, 19 kDa
• GenBank Accession Number: BC009794
• Gene Symbol: VIP
• Gene ID (NCBI): 7432
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vasoactive intestinal peptide (VIP), a short peptide containing 28 amino acids belonging to the secretin-glucagon family, is initially isolated from the gastrointestinal tract as a potent vasodilator peptide. VIP was initially identified in normal nervous tissue and neurons and was subsequently recognized as a neurotransmitter widely distributed in various tissues. The wide distribution of VIP determines its involvement in a range of biological activities, such as gut motility, hormonal regulation, circadian rhythms, immune responses, and carcinogenesis. The general physiologic effects of VIP include vasodilation, anti-inflammatory actions, cell proliferation, hormonal secretion, regulation of gastric motility, and smooth muscle relaxation; therefore, VIP has emerged as a promising drug candidate for the treatment of several diseases.