VNN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

VNN1 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 21745-1-AP

Synonyms

HDLCQ8, Pantetheinase, Pantetheine hydrolase, Tiff66, vanin 1, VNN1

---

Validation Data Gallery

• 

  WB analysis of BxPC-3 using 21745-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 21745-1-AP (VNN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 21745-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 21745-1-AP (VNN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21745-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21745-1-AP (VNN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 21745-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 21745-1-AP (VNN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 21745-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 21745-1-AP (VNN1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 21745-1-AP

  IP result of anti-VNN1 (IP:21745-1-AP, 4ug; Detection:21745-1-AP 1:800) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human pancreas cancer using 21745-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 21745-1-AP (VNN1 Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human pancreas cancer using 21745-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 21745-1-AP (VNN1 Antibody) at dilution of 1:50(under 40x lens).

---

Tested Applications

• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 21745-1-AP is VNN1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VNN1 fusion protein Ag16501
• Full Name: vanin 1
• Calculated Molecular Weight: 513 aa, 57 kDa
• Observed Molecular Weight: 55-72 kDa
• GenBank Accession Number: BC096268
• Gene Symbol: VNN1
• Gene ID (NCBI): 8876
• RRID: AB_10734328
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VNN1(Vascular non-inflammatory molecule 1) is also named as Vanin-1, Pantetheinase, Tiff66. It is a GPI-anchored 70-kDa protein with a high degree of homology with GPI-80 (also known as VNN2), a molecule expressed by human phagocytes and involved in leukocyte adhesion and migration. Vanin-1 is predominantly expressed by resident tissue cells and acts at the level of epithelial cells and surrounding hematopoietic cells by paracrine 2-Mercaptoethylamine release(PMID:17163446). The full length VNN1 has a signal peptide, a propeptide and six glycosylation sites. According to some authors, the variations which varied between 55 and 72kDa may be due to different degrees of glycosylation of the protein and cleavage (JOSE A et al. 2001).

Publications

Species	Application	Title
human	CoIP	Theranostics; Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation.; Authors - Hong Xiang ; KD Validated
mouse	WB	Diabetes; Vanin-1 is a key activator for hepatic gluconeogenesis.; Authors - Siyu Chen
mouse	WB	Sci Signal; Lipid-Induced Toxicity Stimulates Hepatocytes to Release Angiogenic Microparticles That Require Vanin-1 for Uptake by Endothelial Cells.; Authors - Povero Davide D
human	WB	J Lipid Res; VNN1 Promotes Atherosclerosis Progression in apoE Deficiency Mice Fed a High Fat High Cholesterol Diet.; Authors - Yan-Wei Hu
mouse	IHC	J Proteome Res; Novel Elements of the Chondrocyte Stress Response Identified Using an in Vitro Model of Mouse Cartilage Degradation.; Authors - Richard Wilson
mouse	WB	PLoS One; Circulating Extracellular Vesicles with Specific Proteome and Liver MicroRNAs Are Potential Biomarkers for Liver Injury in Experimental Fatty Liver Disease.; Authors - Davide Povero