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  • KD/KO Validated

VNN1 Polyclonal antibody

VNN1 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 21745-1-AP

Synonyms

HDLCQ8, Pantetheinase, Pantetheine hydrolase, Tiff66, vanin 1, VNN1



Tested Applications

Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21745-1-AP is VNN1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VNN1 fusion protein Ag16501
Full Name vanin 1
Calculated molecular weight 513 aa, 57 kDa
Observed molecular weight 55-72 kDa
GenBank accession numberBC096268
Gene symbol VNN1
Gene ID (NCBI) 8876
RRIDAB_10734328
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VNN1(Vascular non-inflammatory molecule 1) is also named as Vanin-1, Pantetheinase, Tiff66. It is a GPI-anchored 70-kDa protein with a high degree of homology with GPI-80 (also known as VNN2), a molecule expressed by human phagocytes and involved in leukocyte adhesion and migration. Vanin-1 is predominantly expressed by resident tissue cells and acts at the level of epithelial cells and surrounding hematopoietic cells by paracrine 2-Mercaptoethylamine release(PMID:17163446). The full length VNN1 has a signal peptide, a propeptide and six glycosylation sites. According to some authors, the variations which varied between 55 and 72kDa may be due to different degrees of glycosylation of the protein and cleavage (JOSE A et al. 2001).

Protocols

Product Specific Protocols
WB protocol for VNN1 antibody 21745-1-APDownload protocol
IHC protocol for VNN1 antibody 21745-1-APDownload protocol
IP protocol for VNN1 antibody 21745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Theranostics

Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation.

Authors - Hong Xiang
  • KD Validated
mouseWB

Diabetes

Vanin-1 is a key activator for hepatic gluconeogenesis.

Authors - Siyu Chen
mouseWB

Sci Signal

Lipid-Induced Toxicity Stimulates Hepatocytes to Release Angiogenic Microparticles That Require Vanin-1 for Uptake by Endothelial Cells.

Authors - Povero Davide D
humanWB

J Lipid Res

VNN1 Promotes Atherosclerosis Progression in apoE Deficiency Mice Fed a High Fat High Cholesterol Diet.

Authors - Yan-Wei Hu
mouseIHC

J Proteome Res

Novel Elements of the Chondrocyte Stress Response Identified Using an in Vitro Model of Mouse Cartilage Degradation.

Authors - Richard Wilson
mouseWB

PLoS One

Circulating Extracellular Vesicles with Specific Proteome and Liver MicroRNAs Are Potential Biomarkers for Liver Injury in Experimental Fatty Liver Disease.

Authors - Davide Povero