Tested Applications
| Positive WB detected in | rat kidney tissue, BxPC-3 cells, mouse kidney tissue, PC-13 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21745-1-AP targets VNN1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16501 Product name: Recombinant human VNN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 302-513 aa of BC096268 Sequence: SQLDSHPSHSAVVNWTSYASSIEALSSGNKEFKGTVFFDEFTFVKLTGVAGNYTVCQKDLCCHLSYKMSENIPNEVYALGAFDGLHTVEGRYYLQICTLLKCKTTNLNTCGDSAETASTRFEMFSLSGTFGTQYVFPEVLLSENQLAPGEFQVSTDGRLFSLKPTSGPVLTVTLFGRLYEKDWASNASSGLTAQARIIMLIVIAPIVCSLSW Predict reactive species |
| Full Name | vanin 1 |
| Calculated Molecular Weight | 513 aa, 57 kDa |
| Observed Molecular Weight | 55-72 kDa |
| GenBank Accession Number | BC096268 |
| Gene Symbol | VNN1 |
| Gene ID (NCBI) | 8876 |
| RRID | AB_10734328 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95497 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VNN1(Vascular non-inflammatory molecule 1) is also named as Vanin-1, Pantetheinase, Tiff66. It is a GPI-anchored 70-kDa protein with a high degree of homology with GPI-80 (also known as VNN2), a molecule expressed by human phagocytes and involved in leukocyte adhesion and migration. Vanin-1 is predominantly expressed by resident tissue cells and acts at the level of epithelial cells and surrounding hematopoietic cells by paracrine 2-Mercaptoethylamine release(PMID:17163446). The full length VNN1 has a signal peptide, a propeptide and six glycosylation sites. According to some authors, the variations which varied between 55 and 72kDa may be due to different degrees of glycosylation of the protein and cleavage (JOSE A et al. 2001).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VNN1 antibody 21745-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VNN1 (Vanin-1) Polyclonal antibody, catalog number 21745-1-AP, has 17 citations. These appear in journals including Theranostics, Diabetes, Science Signaling, Molecular Metabolism, Journal of Lipid Research, Proteomics, PLoS One, Pancreas, Oncology Letters, Frontiers in Pharmacology, Frontiers in Immunology, and others. Research fields span metabolic disease (gluconeogenesis, diabetes, obesity), inflammation and sepsis, atherosclerosis, liver injury, pancreatitis, and cancer (esophageal, renal cell carcinoma), with applications in WB, IHC, IF, and CoIP.
| Species | Application | Title |
|---|---|---|
Theranostics Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation.
| ||
Sci Signal Lipid-Induced Toxicity Stimulates Hepatocytes to Release Angiogenic Microparticles That Require Vanin-1 for Uptake by Endothelial Cells. | ||
Ecotoxicol Environ Saf Integrated toxicological analysis links air pollution-associated acute respiratory distress syndrome signatures to benzo[a]pyrene-induced acute lung injury. | ||
Mol Metab Brown Adipose Vanin-1 is Required for the Maintenance of Mitochondrial Homeostasis and Prevents Diet-Induced Metabolic Dysfunction | ||
Front Pharmacol Integrated analysis of genes shared between type 2 diabetes mellitus and osteoporosis |
