Filter: AllWBIHCIF/ICC

WB analysis using 12804-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12804-1-AP (VPS26A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12804-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12804-1-AP (VPS26A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 12804-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12804-1-AP (VPS26A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12804-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12804-1-AP (VPS26A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 12804-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12804-1-AP (VPS26A antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 12804-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12804-1-AP (VPS26A Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 12804-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12804-1-AP (VPS26A Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HeLa using 12804-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12804-1-AP (VPS26A antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL555-phalloidine stains F-actin (red).
IF Staining of HeLa using 12804-1-AP
Immunofluorescent analysis of HeLa cells using 12804-1-AP (VPS26A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

VPS26A Polyclonal antibody

Name: VPS26A Polyclonal antibody
Brand: Proteintech
SKU: 12804-1-AP
Rating: 4.00 (2 reviews)

VPS26A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12804-1-AP

Publications(8)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

VPS26, Vesicle protein sorting 26A, Vacuolar protein sorting-associated protein 26A, hVPS26

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (8)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse kidney tissue, HEK-293 cells, rat kidney tissue
Positive IHC detected in	human breast cancer tissue, human cervical cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

12804-1-AP targets VPS26A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3391 Product name: Recombinant human VPS26A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 41-327 aa of BC022505 Sequence: LFYDGESVSGKVNLAFKQPGKRLEHQGIRIEFVGQIELFNDKSNTHEFVNLVKELALPGELTQSRSYDFEFMQVEKPYESYIGANVRLRYFLKVTIVRRLTDLVKEYDLIVHQLATYPDVNNSIKMEVGIEDCLHIEFEYNKSKYHLKDVIVGKIYFLLVRIKIQHMELQLIKKEITGIGPSTTTETETIAKYEIMDGAPVKGESIPIRLFLAGYDPTPTMRDVNKKFSVRYFLNLVLVDEEDRRYFKQQEIILWRKAPEKLRKQRTNFHQRFESPESQASAEQPEM Predict reactive species
Full Name	vacuolar protein sorting 26 homolog A (S. pombe)
Calculated Molecular Weight	38 kDa
Observed Molecular Weight	38 kDa
GenBank Accession Number	BC022505
Gene Symbol	VPS26A
Gene ID (NCBI)	9559
RRID	AB_2215033
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75436
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In mammals, there are two paralogues of yeast Vps26, VPS26A and VPS26B (PMID: 16190980). VPS26 is a component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1, and SNX2. VPS26A and VPS26B subunits define distinct retromer complexes (PMID: 21920005). The retromer complex is important in recycling transmembrane receptors from endosomes to the trans-Golgi network (TGN).

Protocols

Product Specific Protocols
IF protocol for VPS26A antibody 12804-1-AP	Download protocol
IHC protocol for VPS26A antibody 12804-1-AP	Download protocol
WB protocol for VPS26A antibody 12804-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cell Metab Phospholipase PLA2G6, a Parkinsonism-Associated Gene, Affects Vps26 and Vps35, Retromer Function, and Ceramide Levels, Similar to α-Synuclein Gain. Authors - Guang Lin View Article
human	WB	bioRxiv Noncanonical roles of ATG5 and membrane atg8ylation in retromer assembly and function Authors - Masroor Ahmad Paddar View Article
human	WB	Elife Noncanonical roles of ATG5 and membrane atg8ylation in retromer assembly and function Authors - Masroor Ahmad Paddar View Article
human	WB,IF	Sci Adv Retromer promotes the lysosomal turnover of mtDNA Authors - Parisa Kakanj View Article
human	WB	Autophagy RAB21 controls autophagy and cellular energy homeostasis by regulating retromer-mediated recycling of SLC2A1/GLUT1 Authors - Yifei Pei View Article
human	WB,IHC	Int J Mol Sci The Prognostic Value and the Oncogenic and Immunological Roles of Vacuolar Protein Sorting Associated Protein 26 A in Pancreatic Adenocarcinoma Authors - Jihuan Hou KD Validated View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Xin (Verified Customer) (10-10-2022)

It is Ok to detect endogenous VPS26A although there are some non-specific bands.

Applications: Western Blot
Primary Antibody Dilution: 1:2000
Cell Tissue Type: mouse brain lysate

VPS26A Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:12804-1-AP)

Stephen (Verified Customer) (09-07-2019)

Oc-2 cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours vps26a antibody incubated 1:300 overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) and Ph647 (red) to visualize DNA and F-actin respectively.