Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 15786-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15786-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 15786-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
rat brain tissue were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 15786-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
K-562 cells were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human urine using 15786-1-AP
human urine sample were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
human urine sample were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 15786-1-AP
IP result of anti-VTA1 (IP:15786-1-AP, 4ug; Detection:15786-1-AP 1:10000) with HEK-293 cells lysate 1440 ug.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15786-1-AP (VTA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15786-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VTA1 antibody (15786-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VTA1 antibody (15786-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HEK-293 cells, A549 cells, HepG2 cells, human urine sample, K-562 cells, rat brain tissue, HSC-T6 cells, SMMC-7721 cells, mouse brain tissue, fetal human brain tissue
Positive IP detected in
HEK-293 cells
Positive IHC detected in
mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:16000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 15786-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15786-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 15786-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 15786-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human urine using 15786-1-AP
human urine sample were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse stomach using 15786-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15786-1-AP (VTA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 15786-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15786-1-AP (VTA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HEK-293 using 15786-1-AP
IP result of anti-VTA1 (IP:15786-1-AP, 4ug; Detection:15786-1-AP 1:10000) with HEK-293 cells lysate 1440 ug.
IF/ICC Figures
IF Staining of HeLa using 15786-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VTA1 antibody (15786-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
VTA1 Polyclonal antibody
Catalog Number
15786-1-AP
Citations
1
Dilutions
WB : 1:2000-1:16000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
