VTA1 Polyclonal antibody

VTA1 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15786-1-AP

Synonyms

C6orf55, DRG 1, DRG1, HSPC228, LIP5, LYST interacting protein 5, My012, SBP1, SKD1 binding protein 1, VTA1

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Validation Data Gallery

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  WB analysis using 15786-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15786-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15786-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human urine using 15786-1-AP

  human urine sample were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15786-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15786-1-AP

  IP result of anti-VTA1 (IP:15786-1-AP, 4ug; Detection:15786-1-AP 1:300) with HEK-293 cells lysate 3000 ug.

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  IF Staining of HeLa using 15786-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VTA1 antibody (15786-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, HepG2 cells, human urine sample, rat brain tissue, fetal human brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

15786-1-AP targets VTA1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VTA1 fusion protein Ag8485
• Full Name: Vps20-associated 1 homolog (S. cerevisiae)
• Calculated molecular weight: 307 aa, 34 kDa
• Observed molecular weight: 34 kDa
• GenBank accession number: BC006989
• Gene symbol: VTA1
• Gene ID (NCBI): 51534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Proteomic analyses uncover a new function and mode of action for mDia2.; Authors - Tadamoto Isogai