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  • KD/KO Validated

VTI1B Polyclonal antibody

VTI1B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14495-1-AP


VTI1, VTI1 LIKE, Vti1 rp1, VTI1B, VTI1L, VTI1L1, VTI2

Tested Applications

Positive WB detected inHEK-293 cells, C6 cells, NIH/3T3 cells, human liver tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14495-1-AP targets VTI1B in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VTI1B fusion protein Ag5906
Full Name vesicle transport through interaction with t-SNAREs homolog 1B (yeast)
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC003142
Gene Symbol VTI1B
Gene ID (NCBI) 10490
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fusion between membranes is mediated by specific SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp which is part of several SNARE complexes in different transport steps (PMID: 12067063). Both proteins had a distinct but overlapping localization. VTI1A is localized predominantly in the TGN, VTI1B in late endosomes (PMID:12067063; 21262811). VTI1B forms a SNARE complex with STX7, STX8 and VAMP8 which functions in the homotypic fusion of late endosomes. It is a component of the SNARE complex composed of STX7, STX8, VAMP7 and VIT1B that is required for heterotypic fusion of late endosomes with lysosomes. It has also been reported that VIT1B interacts with EpsinR, a protein involved in exocytic trafficking (PMID: 15371541).


Product Specific Protocols
WB protocol for VTI1B antibody 14495-1-APDownload protocol
IHC protocol for VTI1B antibody 14495-1-APDownload protocol
IP protocol for VTI1B antibody 14495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




The STX6-VTI1B-VAMP3 complex facilitates xenophagy by regulating the fusion between recycling endosomes and autophagosomes.

Authors - Takashi Nozawa
  • KD Validated


Syntaxin 11 Contributes to the Interferon-Inducible Restriction of Coxiella burnetii Intracellular Infection

Authors - Sandhya Ganesan

Front Cell Dev Biol

The SNARE protein Vti1b is recruited to the sites of BCR activation but is redundant for antigen internalisation, processing and presentation

Authors - Amna Music


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tongbin (Verified Customer) (08-25-2020)

This antibody had a specific band at VTI1B's predicted MW ~28 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart