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  • KD/KO Validated

VTI1B Polyclonal antibody

VTI1B Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14495-1-AP

Synonyms

VTI1, VTI1 LIKE, Vti1 rp1, VTI1B, VTI1L, VTI1L1, VTI2



Tested Applications

Positive WB detected inhuman liver tissue, C6 cells, HeLa cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14495-1-AP targets VTI1B in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VTI1B fusion protein Ag5906
Full Name vesicle transport through interaction with t-SNAREs homolog 1B (yeast)
Calculated molecular weight 27 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC003142
Gene symbol VTI1B
Gene ID (NCBI) 10490
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fusion between membranes is mediated by specific SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp which is part of several SNARE complexes in different transport steps (PMID: 12067063). Both proteins had a distinct but overlapping localization. VTI1A is localized predominantly in the TGN, VTI1B in late endosomes (PMID:12067063; 21262811). VTI1B forms a SNARE complex with STX7, STX8 and VAMP8 which functions in the homotypic fusion of late endosomes. It is a component of the SNARE complex composed of STX7, STX8, VAMP7 and VIT1B that is required for heterotypic fusion of late endosomes with lysosomes. It has also been reported that VIT1B interacts with EpsinR, a protein involved in exocytic trafficking (PMID: 15371541).

Protocols

Product Specific Protocols
WB protocol for VTI1B antibody 14495-1-APDownload protocol
IHC protocol for VTI1B antibody 14495-1-APDownload protocol
IP protocol for VTI1B antibody 14495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

The STX6-VTI1B-VAMP3 complex facilitates xenophagy by regulating the fusion between recycling endosomes and autophagosomes.

Authors - Takashi Nozawa
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tongbin (Verified Customer) (08-25-2020)

This antibody had a specific band at VTI1B's predicted MW ~28 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart