Vinculin Monoclonal antibody

Vinculin Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig and More (2)

Applications

WB, IF, FC, IHC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2B5A7

Cat no : 66305-1-Ig

Synonyms

CMD1W, Metavinculin, MVCL, VCL, Vinculin


Filter:
  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-1-Ig)

    WB analysis using 66305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-1-Ig)

    WB analysis using 66305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Vinculin Monoclonal antibody (66305-1-Ig)

    WB analysis using 66305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig heart tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    WB analysis of pig heart using 66305-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of human breast cancer using 66305-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of human breast cancer using 66305-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of human prostate cancer using 66305-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Vinculin Monoclonal antibody (66305-1-Ig)

    IHC staining of human prostate cancer using 66305-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Vinculin Monoclonal antibody (66305-1-Ig)

    IF Staining of HeLa using 66305-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66305-1-Ig(Vinculin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using Vinculin Monoclonal antibody (66305-1-Ig)

    FC experiment of HeLa using 66305-1-Ig

    1X10^6 HeLa cells were stained with 0.2ug Vinculin antibody (66305-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

"Vinculin Antibodies" Comparison

View side-by-side comparison of Vinculin antibodies from other vendors to find the one that best suits your research needs.

Tested Applications

Positive WB detected inHepG2 cells, pig heart tissue, U-251 cells, LNCaP cells, U-87 MG cells, C6 cells, HEK-293 cells, HeLa cells, HuH-7 cells, L02 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66305-1-Ig targets Vinculin in WB, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, zebrafish, hamster, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vinculin fusion protein Ag24946
Full Name vinculin
Calculated Molecular Weight 1133 aa, 124 kDa
Observed Molecular Weight 117 kDa
GenBank Accession NumberBC039174
Gene Symbol VCL
Gene ID (NCBI) 7414
RRIDAB_2810300
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Protocols

Product Specific Protocols
WB protocol for Vinculin antibody 66305-1-IgDownload protocol
IHC protocol for Vinculin antibody 66305-1-IgDownload protocol
IF protocol for Vinculin antibody 66305-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Crit Care

Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.

Authors - Lingbing Zhang
humanWB

Nat Commun

Modular assembly of the principal microtubule nucleator γ-TuRC.

Authors - Martin Würtz
mouseIF

Circ. Res.

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Authors - Dong Ma
humanWB

Redox Biol

SPTBN2 suppresses ferroptosis in NSCLC cells by facilitating SLC7A11 membrane trafficking and localization

Authors - Jun Deng
humanWB

Cell Death Differ

Loss of RBMS1 promotes anti-tumor immunity through enabling PD-L1 checkpoint blockade in triple-negative breast cancer.

Authors - Jinrui Zhang
humanWB

Cell Death Differ

Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis.

Authors - Weiwei Cui

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Parijat (Verified Customer) (08-21-2023)

Works well as loading control

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293T
FH

Udesh (Verified Customer) (08-16-2023)

Worked well in WB at 1:3000 and IF at 1:100

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Bone Marrow MSCs
FH

Mohamad (Verified Customer) (07-03-2023)

Very good antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
FH

Priya (Verified Customer) (01-17-2023)

I have used for human cardiomyocytes, mouse skin and liver tissues

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cardiomyoctyes, Keratinocytes, Liver and skin
FH

Macarena (Verified Customer) (10-07-2022)

excellent results.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: cell lines
FH

Jonas (Verified Customer) (07-29-2022)

This is my go to loading control stain to validate evenly loaded lanes Binding could be stronger but by using 1:500 dilution, a reliable staining can be achieved

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
Vinculin Antibody Western Blot validation (1:500 dilution) in (Cat no:66305-1-Ig)
FH

Charlotte (Verified Customer) (07-26-2022)

Very good antibody. Very specific, super fast to reveal. Here we see Gli1 (160 kDa) because it is a mouse antibody too.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/5000
  • Cell Tissue Type: NIH-3T3
Vinculin Antibody Western Blot validation (1/5000 dilution) in NIH-3T3 (Cat no:66305-1-Ig)
FH

AKIMASA (Verified Customer) (04-28-2021)

I could get the good quality band!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: OVCAR8, PEO4, Kuramochi, OVSAHO, A2780, HEY,PEO1
FH

Chun (Verified Customer) (09-07-2020)

This is a fairly good antibody for immunoblotting.

  • Applications: Western Blot
  • Cell Tissue Type: HeLa
FH

Huai-Chin (Verified Customer) (06-08-2019)

Serving as a loading control, this antibody is not that good compare to other. Still work to some extent.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: 293T