|Positive WB detected in||HeLa cells, pig heart tissue, rat heart tissue, HepG2 cells, PC-3 cells, A2780 cells, SW 1990 cells, NIH/3T3 cells|
|Positive IHC detected in||human breast cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66305-1-Ig targets Vinculin in WB, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||hamster, human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Vinculin fusion protein Ag24946|
|Calculated molecular weight||1133 aa, 124 kDa|
|Observed molecular weight||117 kDa|
|GenBank accession number||BC039174|
|Gene ID (NCBI)||7414|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.
|Product Specific Protocols|
|WB protocol for Vinculin antibody 66305-1-Ig||Download protocol|
|IHC protocol for Vinculin antibody 66305-1-Ig||Download protocol|
|IF protocol for Vinculin antibody 66305-1-Ig||Download protocol|
|FC protocol for Vinculin antibody 66305-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
AKIMASA (Verified Customer) (04-28-2021)
I could get the good quality band!
Chun (Verified Customer) (09-07-2020)
This is a fairly good antibody for immunoblotting.
Huai-Chin (Verified Customer) (06-08-2019)
Serving as a loading control, this antibody is not that good compare to other. Still work to some extent.