Vinculin Monoclonal antibody

Vinculin Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF, FC, CoIP,ELISA

Conjugate

Unconjugated

CloneNo.

2B5A7

Cat no : 66305-1-Ig

Synonyms

CMD1W, Metavinculin, MVCL, VCL, Vinculin


"Vinculin Antibodies" Comparison

View side-by-side comparison of Vinculin antibodies from other vendors to find the one that best suits your research needs.
  • WB analysis using 66305-1-Ig

    WB analysis using 66305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66305-1-Ig

    IHC staining of human breast cancer using 66305-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 66305-1-Ig

    IHC staining of human breast cancer using 66305-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66305-1-Ig

    IF Staining of HeLa using 66305-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66305-1-Ig(Vinculin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 66305-1-Ig

    FC experiment of HeLa using 66305-1-Ig

    1X10^6 HeLa cells were stained with 0.2ug Vinculin antibody (66305-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.


Tested Applications

Positive WB detected inHeLa cells, pig heart tissue, rat heart tissue, HepG2 cells, PC-3 cells, A2780 cells, SW 1990 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66305-1-Ig targets Vinculin in WB, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity hamster, human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vinculin fusion protein Ag24946
Full Name vinculin
Calculated molecular weight 1133 aa, 124 kDa
Observed molecular weight 117 kDa
GenBank accession numberBC039174
Gene symbol VCL
Gene ID (NCBI) 7414
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Protocols

Product Specific Protocols
WB protocol for Vinculin antibody 66305-1-IgDownload protocol
IHC protocol for Vinculin antibody 66305-1-IgDownload protocol
IF protocol for Vinculin antibody 66305-1-IgDownload protocol
FC protocol for Vinculin antibody 66305-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
hamsterWB,IF

J Mol Neurosci

The Mechanism of Rap1 Regulates N-cadherin to Control Neuronal Migration.

Authors - Ciqing Yang
humanWB

Nucleic Acids Res

BRCA1-associated R-loop affects transcription and differentiation in breast luminal epithelial cells.

Authors - Huai-Chin Chiang
humanWB

Carcinogenesis

Functional genetic variants of RUVBL1 predict overall survival of Chinese patients with epithelial ovarian cancer.

Authors - Haoran Li
ratIF

Can J Physiol Pharmacol

Focal adhesions are involved in simulated microgravity-induced basilar and femoral arterial remodelling in rats.

Authors - Min Jiang
ratIF

Heliyon

The impact of photofunctionalized gold nanoparticles on osseointegration.

Authors - Yassir Elkhidir
humanIF

Cancer Biol Ther

CRMP4a suppresses cell motility by sequestering RhoA activity in prostate cancer cells.

Authors - Changlin Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

AKIMASA (Verified Customer) (04-28-2021)

I could get the good quality band!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: OVCAR8, PEO4, Kuramochi, OVSAHO, A2780, HEY,PEO1
FH

Chun (Verified Customer) (09-07-2020)

This is a fairly good antibody for immunoblotting.

  • Applications: Western Blot
  • Cell Tissue Type: HeLa
FH

Huai-Chin (Verified Customer) (06-08-2019)

Serving as a loading control, this antibody is not that good compare to other. Still work to some extent.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: 293T