Vinculin Monoclonal antibody, PBS Only

Vinculin Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B5A7

Cat no : 66305-1-PBS

Synonyms

CMD1W, Metavinculin, MVCL, VCL, vinculin

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Validation Data Gallery

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  WB analysis using 66305-1-Ig (same clone as 66305-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  WB analysis using 66305-1-Ig (same clone as 66305-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66305-1-Ig (same clone as 66305-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  WB analysis of pig heart using 66305-1-Ig (same clone as 66305-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66305-1-Ig (same clone as 66305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66305-1-Ig (same clone as 66305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66305-1-Ig (same clone as 66305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66305-1-Ig (same clone as 66305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66305-1-Ig (same clone as 66305-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66305-1-Ig(Vinculin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66305-1-Ig (same clone as 66305-1-PBS)

  1X10^6 HeLa cells were stained with 0.2ug Vinculin antibody (66305-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66305-1-PBS targets Vinculin in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Vinculin fusion protein Ag24946
• Full Name: vinculin
• Calculated Molecular Weight: 1133 aa, 124 kDa
• Observed Molecular Weight: 117 kDa
• GenBank Accession Number: BC039174
• Gene Symbol: VCL
• Gene ID (NCBI): 7414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.