CoraLite® Plus 750-conjugated Vinculin Monoclonal antibody

Vinculin Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2B5A7

Cat no : CL750-66305

Synonyms

CMD1W, Metavinculin, MVCL, VCL, vinculin

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Validation Data Gallery

• 

  WB analysis using CL750-66305

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-66305 (Vinculin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-66305 targets Vinculin in WB applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Vinculin fusion protein Ag24946
• Full Name: vinculin
• Calculated Molecular Weight: 1133 aa, 124 kDa
• Observed Molecular Weight: 117 kDa
• GenBank Accession Number: BC039174
• Gene Symbol: VCL
• Gene ID (NCBI): 7414
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.