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Anti-SARS-CoV-2 N protein Human IgG ELISA Kit0 Publications

Catalog number: KE30001

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$399.00

Freight/Packing £00.00

Size: 09µ/gram

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Standard curve of KE30001   Anti-SARS-CoV-2 N protein Human IgG  ELISA Kit

Overview


Product name:
Anti-SARS-CoV-2 N protein Human IgG ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma

Assay type:
Indirect

Range:
8-128 ng/mL

Tested applications:
Indirect ELISA

Product overview:
KE30001 is a qualitative measurement of Anti-SARS-CoV-2 N protein Human IgG in plasma. The principle of the kit is indirect ELISA. N protein recombinant protein has been pre-coated onto microplate well. The samples or standard are added to the well, after incubation the wells are washed and a horseradish peroxidase conjugated anti-Human IgG is added to each well. Producing an complex “Recombinant Protein–human anti-NP IgG antibody-HRP conjugated antibody”. after incubation the wells are washed, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
N protein, nuclepcapsid phosphoprotein
Background

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways . Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. COVID-19 antibodies can be produced by a host immune system following exposure to SARS-CoV-2. IgG and IgM antibodies are also known as immunoglobulins IgG and IgM, respectively, and are among the antibody isotypes produced by vertebrate immune systems. The ELISA microplate is coated with the SARS-CoV-2 nucleocapsid (N) protein. The coated N protein binds with COVID-19 IgG N antibodies in the serum sample.


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