Mouse Vitamin D binding protein Recombinant monoclonal antibody, PBS Only (Capture)

• 

  Sandwich ELISA sample test of MP02757-1

  The mean Vitamin D binding protein concentration was determined to be 2,589.1 ng/mL in mouse eye tissue extract based on a 12.4 mg/mL extract load and 1,046.4 ng/mL in mouse testis tissue extract based on a 1.1 mg/mL extract load.

• 

  Sandwich ELISA sample test of MP02757-1

  Serum of eight mice was measured. The Vitamin D binding protein concentration of detected samples was determined to be 1,971.9 μg/mL with a range of 1,318.6-2,615.8 μg/mL.

• 

  Sandwich ELISA standard curve of MP02757-1

  Sandwich ELISA standard curve of MP02757-1, Mouse Vitamin D binding protein Recombinant Matched Antibody Pair - PBS only. 86884-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg7266. 86884-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Cytometric bead array sample test of MP02757-1

  The mean Vitamin D binding protein concentration was determined to be 932.6 ng/mL in Mouse eye tissue extract based on a 12.4 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP02757-1

  Cytometric bead array standard curve of MP02757-1, MOUSE Vitamin D binding protein Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86884-3-PBS. Detection antibody: 86884-2-PBS. Standard: Eg7266. Range: 0.156-20 ng/mL.

Vitamin D binding protein Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 86884-3-PBS

Clone No.251938C4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

DBP, Gc, Gc-globulin, Group-specific component, VDB

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Product Information

86884-3-PBS targets Vitamin D binding protein as part of a matched antibody pair:

MP02757-1: 86884-3-PBS capture and 86884-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg7266

  Product name: Recombinant mouse Vitamin D BP protein

  Source: mammalian cells-derived, V38

  Tag: N-rFc

  Domain: 17-476 aa of NM_008096.2

  Sequence: LERGRDYEKDKVCNELAMLGKEDFRSLSLILYSRKFSSSTFEQVNQLVKEVVSLTEECCAEGADPTCYDTRTSELSVKSCESDAPFPVHPGTPECCTKEGLERKLCMAALSHQPQEFPTYVEPTNDEICEAFRRDPKGFADQFLYEYSSNYGQAPLPLLVAYTKNYLSMVGSCCTSANPTVCFVKERLQMKHLSLLTTMSNRVCSQYAAYGKEKSRLSHLIKLAQKVPTANLENVLPLAEDFTEILSRCCESTSEDCMASELPEHTIKICQNLSKKNSKFEECCQENTPMNIFMCTYFMPAAEPLQLPAIKLPTGKDLCGQSTTQAMDQYTFELSRRTQVPEVFLSKVLEPTLKTLRECCDTQDSVACFSTQSPLLKRQLTSFIEKGQEMCADYSENTFTEYKKKLAERLRTKTPNTSPAELKDMVEKHSDFASKCCSINSPPLYCSSQIDAEMIDTLQS

• Full Name: group specific component
• Calculated Molecular Weight: 54 kDa
• GenBank Accession Number: NM_008096.2
• Gene Symbol: Gc
• Gene ID (NCBI): 14473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21614
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.