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  • KD/KO Validated

WDR1 Polyclonal antibody

WDR1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13676-1-AP

Synonyms

Actin interacting protein 1, AIP1, NORI 1, WD repeat containing protein 1, WD repeat domain 1, WDR1



Tested Applications

Positive WB detected inHEK-293 cells, rat skeletal muscle tissue, mouse skeletal muscle, Jurkat cells, U-87 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 13676-1-AP is WDR1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR1 fusion protein Ag4670
Full Name WD repeat domain 1
Calculated molecular weight 606 aa, 66 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC030541
Gene symbol WDR1
Gene ID (NCBI) 9948
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for WDR1 antibody 13676-1-APDownload protocol
IHC protocol for WDR1 antibody 13676-1-APDownload protocol
IP protocol for WDR1 antibody 13676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cereb Cortex

Hippocampal Wdr1 Deficit Impairs Learning and Memory by Perturbing F-actin Depolymerization in Mice.

Authors - Jie Wang
mouseWB

Gene

Knockout of Wdr1 results in cardiac hypertrophy and impaired cardiac function in adult mouse heart.

Authors - Xia Huang
  • KO Validated
humanWB

J Biol Chem

Trp-Asp (WD) repeat domain 1 is Essential for Mouse Peri-implantation Development and Regulates Cofilin Phosphorylation.

Authors - Yi Xiao
mouseWB

Dev Biol

WDR1-regulated actin dynamics is required for outflow tract and right ventricle development.

Authors - Jisheng Hu
  • KO Validated
humanWB

Int J Biol Sci

WDR1 Promotes Cell Growth and Migration and Contributes to Malignant Phenotypes of Non-small Cell Lung Cancer through ADF/cofilin-mediated Actin Dynamics.

Authors - Baiyin Yuan
  • KD Validated
human,mouseWB

Mol Cells

WD Repeat Domain 1 Deficiency Inhibits Neointima Formation in Mice Carotid Artery by Modulation of Smooth Muscle Cell Migration and Proliferation.

Authors - JiSheng Hu
  • KO Validated