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- KD/KO Validated
WDR1 Polyclonal antibody
WDR1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 13676-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, rat skeletal muscle tissue, mouse skeletal muscle, Jurkat cells, U-87 cells |
Positive IP detected in | mouse skeletal muscle tissue |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 5 publications below |
WB | See 9 publications below |
Product Information
The immunogen of 13676-1-AP is WDR1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | WDR1 fusion protein Ag4670 |
Full Name | WD repeat domain 1 |
Calculated molecular weight | 606 aa, 66 kDa |
Observed molecular weight | 66-70 kDa |
GenBank accession number | BC030541 |
Gene symbol | WDR1 |
Gene ID (NCBI) | 9948 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for WDR1 antibody 13676-1-AP | Download protocol |
IHC protocol for WDR1 antibody 13676-1-AP | Download protocol |
IP protocol for WDR1 antibody 13676-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Dis AIP1-mediated actin disassembly is required for postnatal germ cell migration and spermatogonial stem cell niche establishment. | ||
Am J Pathol A Cardiomyocyte-Specific Wdr1 Knockout Demonstrates Essential Functional Roles for Actin Disassembly during Myocardial Growth and Maintenance In Mice.
| ||
Mol Cells WD Repeat Domain 1 Deficiency Inhibits Neointima Formation in Mice Carotid Artery by Modulation of Smooth Muscle Cell Migration and Proliferation.
| ||
Int J Biol Sci WDR1 Promotes Cell Growth and Migration and Contributes to Malignant Phenotypes of Non-small Cell Lung Cancer through ADF/cofilin-mediated Actin Dynamics.
| ||
Dev Biol WDR1-regulated actin dynamics is required for outflow tract and right ventricle development.
| ||
J Biol Chem Trp-Asp (WD) repeat domain 1 is Essential for Mouse Peri-implantation Development and Regulates Cofilin Phosphorylation. |