WDR1 Polyclonal antibody

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  WB analysis using 13676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13676-1-AP (WDR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13676-1-AP

  WB result of WDR1 antibody (13676-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR1 transfected HEK-293 cells.

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  WB analysis of rat skeletal muscle using 13676-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13676-1-AP (WDR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 13676-1-AP

  IP result of anti-WDR1 (IP:13676-1-AP, 3ug; Detection:13676-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human testis using 13676-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13676-1-AP (WDR1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 13676-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13676-1-AP (WDR1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 13676-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13676-1-AP (WDR1 antibody) at dilution of 1:100 (under 40x lens).

• Featured Product
• KD/KO Validated

WDR1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13676-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

WD repeat containing protein 1, NORI-1, NORI 1, AIP1, Actin-interacting protein 1

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Tested Applications

• Positive WB detected in: HEK-293 cells, rat skeletal muscle tissue, mouse skeletal muscle, Jurkat cells, U-87 cells
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human colon tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 9 publications below

Product Information

13676-1-AP targets WDR1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR1 fusion protein Ag4670
• Full Name: WD repeat domain 1
• Calculated Molecular Weight: 606 aa, 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC030541
• Gene Symbol: WDR1
• Gene ID (NCBI): 9948
• RRID: AB_10697831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75083
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDR1, which belongs to the WD repeat AIP1 family, also known as AIP1, is a 606 amino acid protein that localizes to the cytoskeleton. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WDR1 induces the disassembly of actin filaments in conjunction with ADF/cofilin family proteins (PMID:15629458, 27557945, 29751004). WDR1 mutations may impair the ability of WDR1 to regulate cofilin-mediated actin depolymerization, and are associated with periodic fever, immunodeficiency, and thrombocytopenia syndrome (PMID: 27557945,27994071,29751004).

Publications

Species	Application	Title
mouse	WB	Cell Death Dis; AIP1-mediated actin disassembly is required for postnatal germ cell migration and spermatogonial stem cell niche establishment.; Authors - J Xu
human	WB	J Biol Chem; Trp-Asp (WD) repeat domain 1 is Essential for Mouse Peri-implantation Development and Regulates Cofilin Phosphorylation.; Authors - Yi Xiao
mouse	WB	Am J Pathol; A Cardiomyocyte-Specific Wdr1 Knockout Demonstrates Essential Functional Roles for Actin Disassembly during Myocardial Growth and Maintenance In Mice.; Authors - Baiyin Yuan ; KO Validated
human,mouse	WB	Mol Cells; WD Repeat Domain 1 Deficiency Inhibits Neointima Formation in Mice Carotid Artery by Modulation of Smooth Muscle Cell Migration and Proliferation.; Authors - JiSheng Hu ; KO Validated
human	WB	Int J Biol Sci; WDR1 Promotes Cell Growth and Migration and Contributes to Malignant Phenotypes of Non-small Cell Lung Cancer through ADF/cofilin-mediated Actin Dynamics.; Authors - Baiyin Yuan ; KD Validated
mouse	WB	Dev Biol; WDR1-regulated actin dynamics is required for outflow tract and right ventricle development.; Authors - Jisheng Hu ; KO Validated