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  • KD/KO Validated

WDR1 Polyclonal antibody

WDR1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 13676-1-AP

Synonyms

Actin interacting protein 1, AIP1, NORI 1, WD repeat containing protein 1, WD repeat domain 1, WDR1



Tested Applications

Positive WB detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 13676-1-AP is WDR1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR1 fusion protein Ag4670
Full Name WD repeat domain 1
Calculated molecular weight 606 aa, 66 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC030541
Gene symbol WDR1
Gene ID (NCBI) 9948
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for WDR1 antibody 13676-1-APDownload protocol
IHC protocol for WDR1 antibody 13676-1-APDownload protocol
IP protocol for WDR1 antibody 13676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cereb Cortex

Hippocampal Wdr1 Deficit Impairs Learning and Memory by Perturbing F-actin Depolymerization in Mice.

Authors - Jie Wang
mouseWB

Gene

Knockout of Wdr1 results in cardiac hypertrophy and impaired cardiac function in adult mouse heart.

Authors - Xia Huang
  • KO Validated
humanWB

J Biol Chem

Trp-Asp (WD) repeat domain 1 is Essential for Mouse Peri-implantation Development and Regulates Cofilin Phosphorylation.

Authors - Yi Xiao
mouseWB

Dev Biol

WDR1-regulated actin dynamics is required for outflow tract and right ventricle development.

Authors - Jisheng Hu
  • KO Validated
humanWB

Int J Biol Sci

WDR1 Promotes Cell Growth and Migration and Contributes to Malignant Phenotypes of Non-small Cell Lung Cancer through ADF/cofilin-mediated Actin Dynamics.

Authors - Baiyin Yuan
  • KD Validated
human,mouseWB

Mol Cells

WD Repeat Domain 1 Deficiency Inhibits Neointima Formation in Mice Carotid Artery by Modulation of Smooth Muscle Cell Migration and Proliferation.

Authors - JiSheng Hu
  • KO Validated