WNK2 Polyclonal antibody

WNK2 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22297-1-AP

Synonyms

Antigen NY CO 43, KIAA1760, NY CO 43, P/OKcl.13, PRKWNK2, SDCCAG43, WNK2

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Validation Data Gallery

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  IHC staining of human heart using 22297-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22297-1-AP (WNK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 22297-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22297-1-AP (WNK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22297-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22297-1-AP (WNK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22297-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22297-1-AP (WNK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 22297-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 22297-1-AP (WNK2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human heart tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22297-1-AP targets WNK2 in IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: WNK lysine deficient protein kinase 2
• Calculated Molecular Weight: 243 kDa
• GenBank Accession Number: NM_006648
• Gene Symbol: WNK2
• Gene ID (NCBI): 65268
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.