WNK4 Recombinant antibody

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  WB analysis of HepG2 using 85886-4-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 85886-4-RR (WNK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85886-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85886-4-RR (WNK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85886-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85886-4-RR against Human WNK4 were performed. The affinity constant is 0.926 nM.

WNK4 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85886-4-RR

Clone No.250156A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:2.7.11.1, PHA2B, PRKWNK4, Protein kinase lysine-deficient 4, Serine/threonine-protein kinase WNK4

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Tested Applications

• Positive WB detected in: HepG2 cells, A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85886-4-RR targets WNK4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: WNK4 fusion protein Ag17773
• Full Name: WNK lysine deficient protein kinase 4
• Calculated Molecular Weight: 1243 aa, 135 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC136664
• Gene Symbol: WNK4
• Gene ID (NCBI): 65266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96J92
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WNK4 (With No Lysine Kinase 4) is a member of the WNK family of serine/threonine protein kinases. It plays a crucial role in regulating electrolyte balance and blood pressure by modulating the activity of ion transporters in the kidney. WNK4 is primarily involved in the regulation of sodium and potassium homeostasis. It achieves this by phosphorylating and activating the kinases SPAK and OSR1, which in turn regulate the activity of the sodium-chloride cotransporter (NCC) in the distal convoluted tubule of the kidney. Additionally, by promoting sodium reabsorption and potassium secretion, WNK4 contributes to the regulation of blood pressure. Increased activity of WNK4 can lead to hypertension. (PMID: 24655003; 33491560)