WNT3A Polyclonal antibody

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  WB analysis of fetal human brain using 26744-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 26744-1-AP (WNT3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26744-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26744-1-AP (WNT3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

WNT3A Polyclonal Antibody for WB, ELISA

Cat No. 26744-1-AP

Publications(40)

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse and More (2)

Applications

WB, ELISA

Synonyms

Protein Wnt 3a, WNT3A

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Tested Applications

• Positive WB detected in: fetal human brain tissue, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 39 publications below

Product Information

26744-1-AP targets WNT3A in WB, ELISA applications and shows reactivity with human, Mouse samples.

• Tested Reactivity: human, Mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WNT3A fusion protein Ag25051
• Full Name: wingless-type MMTV integration site family, member 3A
• Calculated Molecular Weight: 352 aa, 39 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC103921
• Gene Symbol: WNT3A
• Gene ID (NCBI): 89780
• RRID: AB_2918108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P56704
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WNT3A belongs to wingless-type MMTV integration site family, and abnormal Wnt signalling is often associated with severe human diseases, including cancer, osteoporosis and other degenerative disorders (PMID: 20716463). Wnt3a, was shown to promote trophectoderm formation in embryonic stem cells by inducing Cdx2, one of the critical transcription factors of early trophoblast development, in an LEF-1-dependent manner. Wnt3a may also activate other β-catenin-independent cascades such as ERK or PI3K/AKT signalling, again emphasizing the complexity of the Wnt signalling network.

Publications

Species	Application	Title
mouse	WB	Food Funct; Procyanidin B1 and p-coumaric acid from whole highland barley ameliorated HFD-induced impaired glucose tolerance via small intestinal barrier and hepatic glucose metabolism; Authors - Zehua Liu
rat	WB	Aging (Albany NY); Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats.; Authors - Xin Zhang
rat	WB	Arch Toxicol; Developmental exposure to nonylphenol induced rat axonal injury in vivo and in vitro.; Authors - Siyao Li
human	WB	Apoptosis; SKI knockdown suppresses apoptosis and extracellular matrix degradation of nucleus pulposus cells via inhibition of the Wnt/β-catenin pathway and ameliorates disc degeneration.; Authors - Zuo-Long Wu
human	WB	Biomed Pharmacother; BAIAP2L2 promotes the progression of gastric cancer via AKT/mTOR and Wnt3a/β-catenin signaling pathways.; Authors - Jianing Liu
human	WB	J Cancer; Demethylzelasteral inhibits proliferation and EMT via repressing Wnt/β-catenin signaling in esophageal squamous cell carcinoma.; Authors - Jiarui Yu