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  • KD/KO Validated

WWOX Polyclonal antibody

WWOX Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC

Conjugate

Unconjugated

Cat no : 15299-1-AP

Synonyms

D16S432E, FOR, FRA16D, HHCMA56, PRO0128, SDR41C1, WOX1, WWOX



Tested Applications

Positive WB detected inHepG2 cells, mouse skeletal muscle tissue, MCF-7 cells, mouse kidney tissue, mouse liver tissue, mouse testis tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

15299-1-AP targets WWOX in WB, IP, IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WWOX fusion protein Ag7555
Full Name WW domain containing oxidoreductase
Calculated molecular weight 47 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC003184
Gene symbol WWOX
Gene ID (NCBI) 51741
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for WWOX antibody 15299-1-APDownload protocol
IHC protocol for WWOX antibody 15299-1-APDownload protocol
IP protocol for WWOX antibody 15299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncol Lett

Expression and clinical significance of WWOX, Elf5, Snail1 and EMT related factors in epithelial ovarian cancer.

Authors - Yakun Hu
humanWB

Int J Mol Med

WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines.

Authors - Qu Junjie J
  • KD Validated
humanWB

Molecules

Evodiamine Exerts an Anti-Hepatocellular Carcinoma Activity through a WWOX-Dependent Pathway.

Authors - Che-Yuan Hu
humanWB

Hum Mutat

Early infantile onset epileptic encephalopathy 28 due to a homozygous microdeletion involving the WWOX gene in a region of uniparental disomy.

Authors - Mariska Davids