Tested Applications
| Positive WB detected in | HepG2 cells, MCF-7 cells, mouse skeletal muscle tissue, mouse kidney tissue, mouse liver tissue, mouse testis tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human liver cancer tissue, mouse liver tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15299-1-AP targets WWOX in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7555 Product name: Recombinant human WWOX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-234 aa of BC003184 Sequence: MAALRYAGLDDTDSEDELPPGWEERTTKDGWVYYANHTEEKTQWEHPKTGKRKRVAGDLPYGWEQETDENGQVFFVDHINKRTTYLDPRLAFTVDDNPTKPTTRQRYDGSTTAMEILQGRDFTGKVVVVTGANSGIGFETAKSFALHGAHVILACRNMARASEAVSRILEEWQQGAATTVYCAAVPELEGLGGMYFNNCCRCMPSPEAQSEETARTLWALSERLIQERLGSQSG Predict reactive species |
| Full Name | WW domain containing oxidoreductase |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC003184 |
| Gene Symbol | WWOX |
| Gene ID (NCBI) | 51741 |
| RRID | AB_2216389 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZC7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for WWOX antibody 15299-1-AP | Download protocol |
| IP protocol for WWOX antibody 15299-1-AP | Download protocol |
| WB protocol for WWOX antibody 15299-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The WWOX antibody (Cat# 15299-1-AP) has 7 total citations. These appear in journals including International Journal of Molecular Medicine, Biology of Sex Differences, Molecules, International Journal of Cancer, Frontiers in Oncology, Oncology Letters, and Human Mutation. Research fields span cervical cancer, hepatocellular carcinoma, gastric cancer, ovarian cancer, sex development disorders in pigs, and epileptic encephalopathy. Applications include WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Int J Mol Med WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines.
| ||
Biol Sex Differ Whole-genome de novo sequencing reveals genomic variants associated with differences of sex development in SRY negative pigs | ||
Molecules Evodiamine Exerts an Anti-Hepatocellular Carcinoma Activity through a WWOX-Dependent Pathway. | ||
Int J Cancer Remote modulation of WWOX by an intronic variant associated with survival of Chinese gastric cancer patients
| ||
Front Oncol WWOX-rs13338697 genotype predicts therapeutic efficacy of ADI-PEG 20 for patients with advanced hepatocellular carcinoma
| ||
Oncol Lett Expression and clinical significance of WWOX, Elf5, Snail1 and EMT related factors in epithelial ovarian cancer. |
