WWP1 Polyclonal antibody, PBS Only

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  WB analysis of HepG2 using 28689-1-AP (same clone as 28689-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 28689-1-AP (same clone as 28689-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 28689-1-AP (same clone as 28689-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 28689-1-AP (same clone as 28689-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 28689-1-AP (same clone as 28689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 28689-1-AP (same clone as 28689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 28689-1-AP (same clone as 28689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 28689-1-AP (same clone as 28689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

WWP1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 28689-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

AIP5, Atrophin-1-interacting protein 5, EC:2.3.2.26, HECT-type E3 ubiquitin transferase WWP1, hSDRP1

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Product Information

28689-1-PBS targets WWP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30161

  Product name: Recombinant human WWP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC036065

  Sequence: MATASPRSDTSNNHSGRLQLQVTVSSAKLKRKKNWFGTAIYTEVVVDGEITKTAKSSSSSNPKWDEQLTVNVTPQTTLEFQVWSHRTLKADALLGKATIDLKQALLIHNRKLERVKEQLKLSLENKNGIAQTGELTVVLDGLVIEQENITNCSSSPTIEIQENGDALHENGEPSARTTARLAVEGTNGIDNHVPTSTLVQNSCCSYVVNGDNTPSSPSQVAARPKNTPAPKPLASEPADDTVNGESSSFAPTDNASVTGTPVVSEENALSPNCTSTTVEDPPVQEILTSSENNECIPSTSAELESEARSILEPDTSNSRSSSAFEAAKSRQPDGCMDPVRQQSGNANTET

• Full Name: WW domain containing E3 ubiquitin protein ligase 1
• Calculated Molecular Weight: 922 aa, 105 kDa
• Observed Molecular Weight: 95-105 kDa
• GenBank Accession Number: BC036065
• Gene Symbol: WWP1
• Gene ID (NCBI): 11059
• RRID: AB_2881195
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H0M0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.