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XBP1S/XBP1U Polyclonal antibody
XBP1S/XBP1U Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
WB, IHC, ELISA
Cat no : 24168-1-AP
Validation Data Gallery
|Positive WB detected in||HepG2 cells, HEK-293 cells, Jurkat cells, Raji cells|
|Positive IHC detected in||human breast cancer tissue, mouse liver tissue, human colon cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24168-1-AP is XBP1S/XBP1U Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||XBP1S/XBP1U fusion protein Ag21454|
|Full Name||X-box binding protein 1|
|Calculated molecular weight||261 aa, 29 kDa|
|Observed molecular weight||~32 kDa; ~60 kDa|
|GenBank accession number||BC000938|
|Gene ID (NCBI)||7494|
|Purification Method||Antigen Affinity purified|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
XBP1 is also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). IRE1α possesses both kinase and ribonuclease activity and processes XBP1 mRNA to produce an active transcription factor in response to ER stress (PMID: 11779464, 11780124). It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum, the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1. The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1S, which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1U, is constitutively expressed, and thought to function as a negative feedback regulator of XBP1S, which shuts off transcription of target genes during the recovery phase of ER stress (PMID: 11850408). The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The XBP1 antibody (24168-1-AP) can detect both XBP1U and XBP1S.
|Product Specific Protocols|
|WB protocol for XBP1S/XBP1U antibody 24168-1-AP||Download protocol|
|IHC protocol for XBP1S/XBP1U antibody 24168-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Carly (Verified Customer) (09-27-2019)
Used in an antibody array to identify proteins in human EDTA plasma.