|Positive WB detected in||HEK-293 cells, HepG2 cells|
|Positive IHC detected in||human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, mouse liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24168-1-AP is XBP1S/XBP1U Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||XBP1S/XBP1U fusion protein Ag21454|
|Full Name||X-box binding protein 1|
|Calculated molecular weight||261 aa, 29 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC000938|
|Gene ID (NCBI)||7494|
|Purification Method||Antigen Affinity purified|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
XBP1 is also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation(PMID: 14559994). IRE1α possesses both kinase and ribonuclease activity and processes XBP1 mRNA to produce an active transcription factor in response to ER stress (PMID: 11779464, 11780124).It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum , the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1.The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1, which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress(11850408). The XBP1 antibody (24168-1-AP) can detect both isofroms XBP1(U) and XBP1(S).
Cell Death Discov
24(S)-Hydroxycholesterol induces ER dysfunction-mediated unconventional cell death.
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Carly (Verified Customer) (09-27-2019)
Used in an antibody array to identify proteins in human EDTA plasma.