XBP1S/XBP1U Polyclonal antibody

XBP1S/XBP1U Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC

Conjugate

Unconjugated

Cat no : 24168-1-AP

Synonyms

TREB5, X box binding protein 1, XBP 1, XBP1, XBP1s, XBP1S/XBP1U, XBP1U, XBP2



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 24168-1-AP is XBP1S/XBP1U Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XBP1S/XBP1U fusion protein Ag21454
Full Name X-box binding protein 1
Calculated molecular weight 261 aa, 29 kDa
Observed molecular weight ~32 kDa; ~60 kDa
GenBank accession numberBC000938
Gene symbol XBP1
Gene ID (NCBI) 7494
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XBP1 is also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation(PMID: 14559994). IRE1α possesses both kinase and ribonuclease activity and processes XBP1 mRNA to produce an active transcription factor in response to ER stress (PMID: 11779464, 11780124).It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum , the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1.The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1, which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress(11850408). The XBP1 antibody (24168-1-AP) can detect both isofroms XBP1(U) and XBP1(S).

Protocols

Product Specific Protocols
WB protocol for XBP1S/XBP1U antibody 24168-1-APDownload protocol
IHC protocol for XBP1S/XBP1U antibody 24168-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Discov

24(S)-Hydroxycholesterol induces ER dysfunction-mediated unconventional cell death.

Authors - Yasuomi Urano
humanWB

J Exp Clin Cancer Res

Calreticulin promotes EMT in pancreatic cancer via mediating Ca2+ dependent acute and chronic endoplasmic reticulum stress.

Authors - Weiwei Sheng
mouseWB

J Zhejiang Univ Sci B

Endoplasmic reticulum stress is involved in retinal injury induced by repeated transient spikes of intraocular pressure

Authors - Xue YANG

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

  • Applications: Other,