XBP1S/XBP1U Polyclonal antibody

XBP1S/XBP1U Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 24168-1-AP

Synonyms

TREB5, X box binding protein 1, XBP 1, XBP1, XBP1s, XBP1U, XBP2



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 24168-1-AP is XBP1S/XBP1U Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XBP1S/XBP1U fusion protein Ag21454
Full Name X-box binding protein 1
Calculated molecular weight 261 aa, 29 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000938
Gene symbol XBP1
Gene ID (NCBI) 7494
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XBP1 is also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation(PMID: 14559994). IRE1α possesses both kinase and ribonuclease activity and processes XBP1 mRNA to produce an active transcription factor in response to ER stress (PMID: 11779464, 11780124).It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum , the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1.The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1, which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress(11850408). The XBP1 antibody (24168-1-AP) can detect both isofroms XBP1(U) and XBP1(S).

Protocols

Product Specific Protocols
WB protocol for XBP1S/XBP1U antibody 24168-1-APDownload protocol
IHC protocol for XBP1S/XBP1U antibody 24168-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Discov

24(S)-Hydroxycholesterol induces ER dysfunction-mediated unconventional cell death.

Authors - Yasuomi Urano
humanWB

J Exp Clin Cancer Res

Calreticulin promotes EMT in pancreatic cancer via mediating Ca2+ dependent acute and chronic endoplasmic reticulum stress.

Authors - Weiwei Sheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

  • Applications: Other,