XCL1 Polyclonal antibody

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  IHC staining of mouse spleen using 33408-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 33408-1-AP (XCL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 33408-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 33408-1-AP (XCL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 33408-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 33408-1-AP (XCL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 33408-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 33408-1-AP (XCL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

XCL1 Polyclonal Antibody for IHC, ELISA

Cat No. 33408-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, ELISA

Synonyms

C motif chemokine 1, Cytokine SCM-1, Lptn, Ltn, Lymphotactin

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Tested Applications

• Positive IHC detected in: mouse spleen tissue, mouse thymus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33408-1-AP targets XCL1 in IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg2932

  Product name: recombinant mouse Xcl1 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 22-114 aa of NM_008510.1

  Sequence: VGTEVLEESSCVNLQTQRLPVQKIKTYIIWEGAMRAVIFVTKRGLKICADPEAKWVKAAIKTVDGRASTRKNMAETVPTGAQRSTSTAITLTG

• Full Name: chemokine (C motif) ligand 1
• Calculated Molecular Weight: 12 kDa
• GenBank Accession Number: NM_008510.1
• Gene Symbol: Xcl1
• Gene ID (NCBI): 16963
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P47993
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XCL1/lymphotactin is a member of the chemokine family, which is expressed by immune cells, including activated CD8+ T cells, CD4‏+ T cells, natural killer cells (NKCs), and natural killer T (NKT) cells. XCR1, the XCL1 receptor, is a member of the C-chemokine subfamily expressed on CD4+ and CD8+ T cells, NKCs, B cells, and neutrophils. XCL1 has a potential role in inflammatory diseases, including chronic inflammatory bowel disease, autoimmune diabetes, encephalomyelitis, and rheumatoid arthritis (RA).