XDH Polyclonal antibody

• Featured Product
• KD/KO Validated

XDH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 55156-1-AP

Synonyms

xanthine dehydrogenase, Xanthine Oxidase, Xanthine oxidoreductase, XD, XDH, XDHA, XO, XOR

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Validation Data Gallery

• 

  WB analysis of mouse liver using 55156-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55156-1-AP (XDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55156-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55156-1-AP (XDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55156-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55156-1-AP (XDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 55156-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 55156-1-AP (XDH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 55156-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55156-1-AP (XDH antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 55156-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55156-1-AP (XDH antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 55156-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55156-1-AP (XDH antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 55156-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55156-1-AP (XDH antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 20 publications below

Product Information

55156-1-AP targets XDH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: xanthine dehydrogenase
• Calculated Molecular Weight: 146 kDa
• Observed Molecular Weight: 147-150 kDa
• GenBank Accession Number: NM_000379
• Gene Symbol: XDH
• Gene ID (NCBI): 7498
• RRID: AB_10860905
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XDH, also named as XDHA, XO and XD, belongs to the xanthine dehydrogenase family. It catalyzes the last two steps of purine catabolism in man, formation of the end product uric acid from hypoxanthine and xanthine. The mammalian enzyme is synthesized as a dehydrogenase (XDH), which uses NAD as the electron acceptor, but it can be converted into an oxidase (XO) both in vivo and in vitro.This gene encodes a protein of 150 kDa and the 150 kDa polypeptide can be processed into a fragment of 130 kDa and further to 85 kDa by spontaneous proteolytic cleavage(PMID:9989587). In addition, the full length polypeptide can be also partially processed into a 87 kDa and 59 kDa fragments(PMID:21528298). It also can exsit as a homodimer(PMID:9989587). This antibody is specific to XDH.

Publications

Species	Application	Title
mouse,human	WB	Int J Mol Sci; Multiple-Purpose Connectivity Map Analysis Reveals the Benefits of Esculetin to Hyperuricemia and Renal Fibrosis.; Authors - Yiming Wang
mouse	WB	Free Radic Biol Med; PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.; Authors - Xue Chen
mouse	WB	Nutrients; Chinese Sumac (Rhus chinensis Mill.) Fruits Prevent Hyperuricemia and Uric Acid Nephropathy in Mice Fed a High-Purine Yeast Diet; Authors - Nan Ma
mouse	WB	Biochem Pharmacol; PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1.; Authors - Yunhui Xu
mouse	WB	Sci Rep; Iron regulatory protein deficiency compromises mitochondrial function in murine embryonic fibroblasts.; Authors - Huihui Li
chicken	WB	Poult Sci; Effects of Smilax China L. extracts on Hyperuricemia chicken model via inhibiting xanthine oxidase activity; Authors - Mingen Yan