Tested Applications
| Positive WB detected in | LNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells |
| Positive IHC detected in | human colon tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue, human colon tissue |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66800-1-Ig targets XIAP in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18088 Product name: Recombinant human XIAP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-497 aa of BC032729 Sequence: MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSASTLARAGFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAMYSEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNWEPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKIMEEKIQISGSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLCKICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITFKQKIFMS Predict reactive species |
| Full Name | X-linked inhibitor of apoptosis |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC032729 |
| Gene Symbol | XIAP |
| Gene ID (NCBI) | 331 |
| RRID | AB_2882143 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P98170 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for XIAP antibody 66800-1-Ig | Download protocol |
| IF protocol for XIAP antibody 66800-1-Ig | Download protocol |
| IHC protocol for XIAP antibody 66800-1-Ig | Download protocol |
| WB protocol for XIAP antibody 66800-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-XIAP antibody (catalog no. 66800-1-Ig) has 17 total citations. These appear in journals including Nature Nanotechnology, Nature Chemical Biology, Advanced Science, Cell Death & Disease, Cell Death & Differentiation, International Journal of Biological Sciences, JACC Basic Translational Science, Antioxidants, Cancer Gene Therapy, International Journal of Pharmaceutics, International Journal of Molecular Sciences, Chemical & Biological Interactions, Toxicology Research, Gene, Oncology Letters, Biotechnology & Histochemistry, and bioRxiv. Research fields span cancer biology, apoptosis, neurodegeneration, inflammation, and gene editing.
| Species | Application | Title |
|---|---|---|
Nat Nanotechnol Non-invasive activation of intratumoural gene editing for improved adoptive T-cell therapy in solid tumours | ||
Adv Sci (Weinh) XIAP Stabilizes DDRGK1 to Promote ER-Phagy and Protects Against Noise-Induced Hearing Loss.
| ||
Int J Biol Sci Inhibition of PI3K/AKT signaling via ROS regulation is involved in Rhein-induced apoptosis and enhancement of oxaliplatin sensitivity in pancreatic cancer cells. | ||
JACC Basic Transl Sci Bag3 Regulates Mitochondrial Function and the Inflammasome Through Canonical and Noncanonical Pathways in the Heart |
Reviews
What customers say
Both reviewers gave this antibody a 5-star rating, describing it as a "very good antibody." The reviews were based on use in immunoprecipitation experiments with AC16 cells. Overall, customers expressed satisfaction with the product's performance for IP applications, with no negative feedback or technical issues reported.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Praveen (Verified Customer) (05-17-2019) | Very Good Antibody
 |
Praveen (Verified Customer) (05-17-2019) | Very good Antibody
 |
