XIAP Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 66800-1-Ig (same clone as 66800-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66800-1-Ig (XIAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66800-1-Ig (same clone as 66800-1-PBS)

  WB result of XIAP antibody (66800-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XIAP transfected HEK-293 cells. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66800-1-Ig (same clone as 66800-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66800-1-Ig (XIAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using XIAP antibody (66800-1-Ig, Clone: 2A1A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using XIAP antibody (66800-1-Ig, Clone: 2A1A12 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using XIAP antibody (66800-1-Ig, Clone: 2A1A12 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66800-1-Ig (same clone as 66800-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human XIAP (66800-1-Ig, Clone:2A1A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51614-1

  Cytometric bead array sample test of MP51614-1, XIAP Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66800-1-PBS. Detection antibody: 66800-2-PBS.

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  Cytometric bead array standard curve of MP51614-1

  Cytometric bead array standard curve of MP51614-1, XIAP Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66800-1-PBS. Detection antibody: 66800-2-PBS. Standard:Ag18088. Range: 3.125-400 ng/mL.

• Featured Product
• KD/KO Validated

XIAP Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Cat No. 66800-1-PBS

Clone No.2A1A12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

2A1A12, API3, Baculoviral IAP repeat-containing protein 4, E3 ubiquitin-protein ligase XIAP, EC:2.3.2.27

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Product Information

66800-1-PBS targets XIAP as part of a matched antibody pair:

MP51614-1: 66800-1-PBS capture and 66800-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18088

  Product name: Recombinant human XIAP protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-497 aa of BC032729

  Sequence: MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSASTLARAGFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAMYSEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNWEPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKIMEEKIQISGSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLCKICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITFKQKIFMS

• Full Name: X-linked inhibitor of apoptosis
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC032729
• Gene Symbol: XIAP
• Gene ID (NCBI): 331
• RRID: AB_2882143
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P98170
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells.