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- KD/KO Validated
XRCC5/Ku80 Polyclonal antibody
XRCC5/Ku80 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 16389-1-AP
|Positive WB detected in||HepG2 cells, K-562 cells, HEK-293 cells, A431 cells, human liver tissue, HeLa cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human colon cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
16389-1-AP targets XRCC5/Ku80 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||XRCC5/Ku80 fusion protein Ag9454|
|Full Name||X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)|
|Calculated molecular weight||732 aa, 83 kDa|
|Observed molecular weight||80 kDa|
|GenBank accession number||BC019027|
|Gene ID (NCBI)||7520|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication. Thus, Ku80 and Ku86 are the same protein.
|Product Specific Protocols|
|WB protocol for XRCC5/Ku80 antibody 16389-1-AP||Download protocol|
|IHC protocol for XRCC5/Ku80 antibody 16389-1-AP||Download protocol|
|IF protocol for XRCC5/Ku80 antibody 16389-1-AP||Download protocol|
|IP protocol for XRCC5/Ku80 antibody 16389-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Cell Death Dis
OGA is associated with deglycosylation of NONO and the KU complex during DNA damage repair.
Flavone protects HBE cells from DNA double-strand breaks caused by PM2.5.
Elevated XRCC5 expression level can promote temozolomide resistance and predict poor prognosis in glioblastoma.
Downregulation of Mitochondrial Single Stranded DNA Binding Protein (SSBP1) Induces Mitochondrial Dysfunction and Increases the Radiosensitivity in Non-Small Cell Lung Cancer Cells.