XRCC5/Ku80 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 16389-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 41 μg/150 μl

Choose size:

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Species specificity:
human

Positive WB detected in:
HepG2 cells, K-562 cells, HEK-293 cells, A431 cells, human liver tissue, HeLa cells

Positive IP detected in:
HEK-293 cells

Positive IHC detected in:
human colon cancer tissue, human colon cancer tissue, human lung cancer tissue

Positive IF detected in:
HepG2 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)

Calculated molecular weight:
732aa,83 kDa

Observed molecular weight:
80 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
XRCC5

Synonyms
86 kDa subunit of Ku antigen, CTC85, CTCBF, DNA repair protein XRCC5, FLJ39089, G22P2, KARP 1, KARP1, Ku80, Ku86, KUB2, NFIV, Nuclear factor IV, Thyroid lupus autoantigen, TLAA, XRCC5
Background

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication. Thus, Ku80 and Ku86 are the same protein.


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