|Positive WB detected in||HepG2 cells, K-562 cells, HEK-293 cells, A431 cells, human liver tissue, HeLa cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human colon cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
16389-1-AP targets XRCC5/Ku80 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||XRCC5/Ku80 fusion protein Ag9454|
|Full Name||X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)|
|Calculated molecular weight||732 aa, 83 kDa|
|Observed molecular weight||80 kDa|
|GenBank accession number||BC019027|
|Gene ID (NCBI)||7520|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication. Thus, Ku80 and Ku86 are the same protein.
|Product Specific Protocols|
|WB protocol for XRCC5/Ku80 antibody 16389-1-AP||Download protocol|
|IHC protocol for XRCC5/Ku80 antibody 16389-1-AP||Download protocol|
|IF protocol for XRCC5/Ku80 antibody 16389-1-AP||Download protocol|
|IP protocol for XRCC5/Ku80 antibody 16389-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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