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  • KD/KO Validated

XRCC5/Ku80 Polyclonal antibody

XRCC5/Ku80 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 16389-1-AP

Synonyms

86 kDa subunit of Ku antigen, CTC85, CTCBF, DNA repair protein XRCC5, G22P2, KARP 1, KARP1, Ku80, Ku86, KUB2, NFIV, Nuclear factor IV, Thyroid lupus autoantigen, TLAA, XRCC5, XRCC5/Ku80



Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, HEK-293 cells, A431 cells, human liver tissue, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16389-1-AP targets XRCC5/Ku80 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XRCC5/Ku80 fusion protein Ag9454
Full Name X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
Calculated molecular weight 732 aa, 83 kDa
Observed molecular weight 80-83 kDa
GenBank accession numberBC019027
Gene symbol XRCC5
Gene ID (NCBI) 7520
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication. Thus, Ku80 and Ku86 are the same protein.

Protocols

Product Specific Protocols
WB protocol for XRCC5/Ku80 antibody 16389-1-APDownload protocol
IHC protocol for XRCC5/Ku80 antibody 16389-1-APDownload protocol
IF protocol for XRCC5/Ku80 antibody 16389-1-APDownload protocol
IP protocol for XRCC5/Ku80 antibody 16389-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IP

Immunity

Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.

Authors - Yan Wang
humanIF

Oncogene

UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.

Authors - L Hu
humanWB

Oncotarget

Sensitization of tamoxifen-resistant breast cancer cells by Z-ligustilide through inhibiting autophagy and accumulating DNA damages.

Authors - Hongyi Qi
mouseIF

Sci Rep

Double Strand Break DNA Repair occurs via Non-Homologous End-Joining in Mouse MII Oocytes.

Authors - Jacinta H Martin
humanWB

Front Oncol

ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway

Authors - Xin Wen
humanWB,CoIP

iScience

WASH interacts with Ku to regulate DNA double-stranded break repair.

Authors - Tao Wang
  • KD Validated