XRN1 Polyclonal antibody

XRN1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 23108-1-AP

Synonyms

5 3 exoribonuclease 1, 5' 3' exoribonuclease 1, SEP1, XRN1



Tested Applications

Positive WB detected inHeLa cells, Neuro-2a cells
Positive IHC detected inhuman heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23108-1-AP is XRN1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XRN1 fusion protein Ag19398
Full Name 5'-3' exoribonuclease 1
Calculated molecular weight 1706 aa, 194 kDa
Observed molecular weight 175 kDa
GenBank accession numberBC078146
Gene symbol XRN1
Gene ID (NCBI) 54464
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Exoribonuclease I (XRN1), also known as Sep1 or Rar5, is a 1,694-amino acid protein that functions as the major cytoplasmic 5 prime to 3 prime exoribonuclease and plays an important role in mRNA turnover. XRN1 may also function in the microtubular cytoskeleton as well as in DNA recombination and replication. XRN1 induces the expression of stress granules (SGs), cytoplasmic aggregates of stalled translational preinitiation complexes that accumulate during stress, and GW bodies/processing bodies (PBs), distinct cytoplasmic sites of mRNA degradation. There are several isoforms of XRN1 that are produced as a result of alternative splicing events.Loss of XRN1 markedly affects cell growth rates.This antibody is specific to the XRN1 protein.

Protocols

Product Specific Protocols
WB protocol for XRN1 antibody 23108-1-APDownload protocol
IHC protocol for XRN1 antibody 23108-1-APDownload protocol
IF protocol for XRN1 antibody 23108-1-APDownload protocol
Standard Protocols
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