YM-1+YM-2 Polyclonal antibody

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  WB analysis using 33153-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33153-1-AP (YM-1+YM-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

YM-1+YM-2 Polyclonal Antibody for WB, ELISA

Cat No. 33153-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Beta-N-acetylhexosaminidase Ym1, Chi3l3, Chil3, CHIL4, Chitinase-3-like protein 3

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Tested Applications

• Positive WB detected in: mouse bone marrow, mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33153-1-AP targets YM-1+YM-2 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag39745

  Product name: Recombinant mouse CHIL3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 22-368 aa of NM_009892

  Sequence: YQLMCYYTSWAKDRPIEGSFKPGNIDPCLCTHLIYAFAGMQNNEITYTHEQDLRDYEALNGLKDKNTELKTLLAIGGWKFGPAPFSAMVSTPQNRQIFIQSVIRFLRQYNFDGLNLDWQYPGSRGSPPKDKHLFSVLVKEMRKAFEEESVEKDIPRLLLTSTGAGIIDVIKSGYKIPELSQSLDYIQVMTYDLHDPKDGYTGENSPLYKSPYDIGKSADLNVDSIISYWKDHGAASEKLIVGFPAYGHTFILSDPSKTGIGAPTISTGPPGKYTDESGLLAYYEVCTFLNEGATEVWDAPQEVPYAYQGNEWVGYDNVRSFKLKAQWLKDNNLGGAVVWPLDMDDFSGSFCHQRHFPLTSTLKGDLNIHSASCKGPY

• Full Name: chitinase 3-like 3
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: NM_009892
• Gene Symbol: Chi3l3
• Gene ID (NCBI): 12655
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O35744
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chitinase-like protein 3 (Chil3), also called ECF-L or Ym1, is a member of chitinase-like protein family which has chemotactic activity for eosinophils (PMID: 27256220). CHIL3 promotes NSC self-renewal and commitment to the neuronal lineage. Endogenous expression of CHIL3 is observed in the neurogenic niche of the mouse brain and retina and in lesions of those injury models (PMID: 36368330). Chitinase-like protein 4 (Chil4), also called Ym2, regulates regeneration of the injured OE in vivo in both male and female mice and cell proliferation/differentiation in OE colonies in vitro (PMID: 34016714). This antibody detects both YM1 and YM2.