Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HCT 116 cells, HepG2 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells, C6 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25537-1-AP targets YTHDF3-specific in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22374 Product name: Recombinant human YTHDF3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 284-351 aa of BC052970 Sequence: SVVKAPPTQPVLPPQTIIQQPQPLIQPPPLVQSQLPQQQPQPPQPQQQQGPQPQAQPHQVQPQQQQLQ Predict reactive species |
| Full Name | YTH domain family, member 3 |
| Calculated Molecular Weight | 585 aa, 64 kDa |
| Observed Molecular Weight | 64 kDa |
| GenBank Accession Number | BC052970 |
| Gene Symbol | YTHDF3 |
| Gene ID (NCBI) | 253943 |
| RRID | AB_2847817 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z739 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Our antibody 25537-1-AP specially reacts with YTHDF3 protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for YTHDF3-specific antibody 25537-1-AP | Download protocol |
| IHC protocol for YTHDF3-specific antibody 25537-1-AP | Download protocol |
| WB protocol for YTHDF3-specific antibody 25537-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The YTHDF3-specific Polyclonal antibody (Cat# 25537-1-AP) has 156 citations spanning high-impact journals including Nature, Science, Nature Communications, Cell Reports, Molecular Cell, Nature Cancer, Cell Research, and Oncogene, among others. Research fields covered include m6A RNA modification, cancer biology (colorectal, breast, glioblastoma, hepatocellular, gastric, and lung cancers), neurodegeneration, viral infection, metabolism, immunology, fibrosis, cardiovascular disease, stem cell biology, and aging.
| Species | Application | Title |
|---|---|---|
Science Liver ALKBH5 regulates glucose and lipid homeostasis independently through GCGR and mTORC1 signaling | ||
Mol Cancer A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis
| ||
Mol Cancer CircZFR/YTHDF3 axis drives lymph node metastasis in cervical cancer via FASN translation | ||
Cell Res Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells. | ||
Cancer Commun (Lond) Targeting histone deacetylase suppresses tumor growth through eliciting METTL14-modified m6 A RNA methylation in ocular melanoma |
Reviews
What customers say
All three reviews rate this antibody 5 stars. Users report strong, specific signal in Western blot at 1:1000 dilution across human (HeLa, SH-SY5Y), mouse, and porcine cells. Immunofluorescence/ICC shows clear cytoplasmic staining and recognition of stress granules, while IHC yields specific signal in mouse spinal cord with both DAB and fluorescence. One user confirmed knockdown specificity using YTHDF3 siRNA. Overall, the antibody is well-regarded for consistent performance across multiple applications and species.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tatyana (Verified Customer) (12-22-2024) | Antibody works in WB, ICC and IHC. ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Cytoplasmic staining and recognises stress granules. WB with antibody diluted in 4% milk in TBST, ON incubation. Also gave what looks like specific signal in mouse spinal cord (IHC, both DAB and fluorescence).
 |
Roy (Verified Customer) (06-15-2024) | Strong signal for YTHDF3 with this antibody from HeLa extracts (1h incubation at room temperature, 1/1000 dilution)
|
Robert (Verified Customer) (01-17-2019) | 1. scrambled siRNA2. YTHDF3 siRNA
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