YTHDF3-specific Polyclonal antibody

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  WB analysis of HeLa using 25537-1-AP

  WB result of YTHDF3-specific antibody (25537-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDF3-specific transfected HeLa cells.

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  WB analysis using 25537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25537-1-AP (YTHDF3-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 25537-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25537-1-AP (YTHDF3-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25537-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25537-1-AP (YTHDF3-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-12 using 25537-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using YTHDF3-specific antibody (25537-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

YTHDF3-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25537-1-AP

Publications(120)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

YTHDF3, DF3, YTH domain-containing family protein 3

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HCT 116 cells, HepG2 cells, PC-12 cells, NIH/3T3 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 25 publications below
• WB: See 99 publications below
• IHC: See 6 publications below
• IF: See 12 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below
• RIP: See 17 publications below

Product Information

25537-1-AP targets YTHDF3-specific in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22374

  Product name: Recombinant human YTHDF3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 284-351 aa of BC052970

  Sequence: SVVKAPPTQPVLPPQTIIQQPQPLIQPPPLVQSQLPQQQPQPPQPQQQQGPQPQAQPHQVQPQQQQLQ

• Full Name: YTH domain family, member 3
• Calculated Molecular Weight: 585 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC052970
• Gene Symbol: YTHDF3
• Gene ID (NCBI): 253943
• RRID: AB_2847817
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z739
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Our antibody 25537-1-AP specially reacts with YTHDF3 protein.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nature; m6A facilitates hippocampus-dependent learning and memory through YTHDF1.; Authors - Hailing Shi
human	WB,CoIP	Mol Cancer; A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis; Authors - Kaixuan Zeng ; KD Validated
mouse	WB,RIP	Cell Mol Immunol; Inhibition of METTL3 in macrophages provides protection against intestinal inflammation; Authors - Huilong Yin
human	WB,IF	Nat Cell Biol; O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins; Authors - Yulin Chen
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
human	WB	Nat Commun; Circular RNA encoded MET variant promotes glioblastoma tumorigenesis; Authors - Jian Zhong ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (12-22-2024)

Antibody works in WB, ICC and IHC. ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Cytoplasmic staining and recognises stress granules. WB with antibody diluted in 4% milk in TBST, ON incubation. Also gave what looks like specific signal in mouse spinal cord (IHC, both DAB and fluorescence).

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa and SH-SY5Y cells/human; mouse tissue

YTHDF3-specific Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in HeLa and SH-SY5Y cells/human; mouse tissue (Cat no:25537-1-AP)

FH

Roy (Verified Customer) (06-15-2024)

Strong signal for YTHDF3 with this antibody from HeLa extracts (1h incubation at room temperature, 1/1000 dilution)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

FH

Robert (Verified Customer) (01-17-2019)

1. scrambled siRNA2. YTHDF3 siRNA

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: ST cells (porcine)

YTHDF3-specific Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:25537-1-AP)