CoraLite®488-conjugated YY1 Monoclonal antibody

YY1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, monkey

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2E11C5

Cat no : CL488-66281

Synonyms

INO80S, UCRBP, YY1

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66281

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66281 (YY1 antibody) at dilution of 1:50 ..

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66281 targets YY1 in IF applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: YY1 fusion protein Ag17732
• Full Name: YY1 transcription factor
• Calculated Molecular Weight: 414 aa, 45 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC037308
• Gene Symbol: YY1
• Gene ID (NCBI): 7528
• RRID: AB_2883302
• Conjugate: CoraLite®488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 491 nm / 516 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.