CoraLite®488-conjugated YY1 Monoclonal antibody

YY1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, monkey

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2E11C5

Cat no : CL488-66281

Synonyms

INO80S, UCRBP, YY1



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66281-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66281 targets YY1 in IF applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen YY1 fusion protein Ag17732
Full Name YY1 transcription factor
Calculated molecular weight 414 aa, 45 kDa
GenBank accession numberBC037308
Gene symbol YY1
Gene ID (NCBI) 7528
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65–68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL488 YY1 antibody CL488-66281Download protocol
Standard Protocols
Click here to view our Standard Protocols