YY1 Monoclonal antibody
YY1 Monoclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, monkey
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
CloneNo.
2E11C5
Cat no : 66281-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | C6 cells, HeLa cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 9 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
66281-1-Ig targets YY1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | YY1 fusion protein Ag17732 |
| Full Name | YY1 transcription factor |
| Calculated molecular weight | 414 aa, 45 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC037308 |
| Gene symbol | YY1 |
| Gene ID (NCBI) | 7528 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65–68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for YY1 antibody 66281-1-Ig | Download protocol |
| IHC protocol for YY1 antibody 66281-1-Ig | Download protocol |
| IF protocol for YY1 antibody 66281-1-Ig | Download protocol |
| IP protocol for YY1 antibody 66281-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer. | ||
Mol Cancer CircPSMC3 suppresses the proliferation and metastasis of gastric cancer by acting as a competitive endogenous RNA through sponging miR-296-5p. | ||
J Cell Mol Med FAM3C-YY1 axis is essential for TGFβ-promoted proliferation and migration of human breast cancer MDA-MB-231 cells via the activation of HSF1. | ||
Prostate hnRNPM, a potential mediator of YY1 in promoting the epithelial-mesenchymal transition of prostate cancer cells. | ||
Environ Toxicol Transcription factor YY1 enhances the stemness of lung cancer cells by stabilizing hypoxia factor HIF-1α under a hypoxic microenvironment. | ||
Stem Cell Res MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1. |
