YY1 Monoclonal antibody

YY1 Monoclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E11C5

Cat no : 66281-1-Ig

Synonyms

DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor


"YY1 Antibodies" Comparison

View side-by-side comparison of YY1 antibodies from other vendors to find the one that best suits your research needs.
  • IF Staining of HepG2 using 66281-1-Ig

    IF Staining of HepG2 using 66281-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66281-1-Ig (YY1 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HeLa using 66281-1-Ig

    WB analysis of HeLa using 66281-1-Ig

    Western blot of YY1 in various human cell lines with 66281-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of C6 using 66281-1-Ig

    WB analysis of C6 using 66281-1-Ig

    Western blot of YY1 in rat (C6 and ROS1728) and mouse(NIH3T3 and Raw264.7) cell lines with 66281-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IP experiment of NIH/3T3 using 66281-1-Ig

    IP experiment of NIH/3T3 using 66281-1-Ig

    IP Result of anti-YY1 (IP:66281-1-Ig, 5ug; Detection:66281-1-Ig 1:1000) with NIH/3T3 cells lysate 4000ug.

  • IHC staining of human breast cancer using 66281-1-Ig

    IHC staining of human breast cancer using 66281-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66281-1-Ig (YY1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).


Tested Applications

Positive WB detected inC6 cells, HeLa cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66281-1-Ig targets YY1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen YY1 fusion protein Ag17732
Full Name YY1 transcription factor
Calculated molecular weight 414 aa, 45 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC037308
Gene symbol YY1
Gene ID (NCBI) 7528
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65–68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.

Protocols

Product Specific Protocols
WB protocol for YY1 antibody 66281-1-IgDownload protocol
IHC protocol for YY1 antibody 66281-1-IgDownload protocol
IF protocol for YY1 antibody 66281-1-IgDownload protocol
IP protocol for YY1 antibody 66281-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Nat Commun

The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer.

Authors - Weimei Tang
WB

Mol Cancer

CircPSMC3 suppresses the proliferation and metastasis of gastric cancer by acting as a competitive endogenous RNA through sponging miR-296-5p.

Authors - Dawei Rong
humanWB

J Cell Mol Med

FAM3C-YY1 axis is essential for TGFβ-promoted proliferation and migration of human breast cancer MDA-MB-231 cells via the activation of HSF1.

Authors - Weili Yang
humanWB,IHC

Prostate

hnRNPM, a potential mediator of YY1 in promoting the epithelial-mesenchymal transition of prostate cancer cells.

Authors - Tong Yang

Environ Toxicol

Transcription factor YY1 enhances the stemness of lung cancer cells by stabilizing hypoxia factor HIF-1α under a hypoxic microenvironment.

Authors - Xianping Huang
mouseWB

Stem Cell Res

MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1.

Authors - Min Jin