YY1 Monoclonal antibody

YY1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

18

CloneNo.

2E11C5

Cat no : 66281-1-Ig

Synonyms

DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor

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• Validation Data Gallery

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  WB analysis of HeLa using 66281-1-Ig

  Western blot of YY1 in various human cell lines with 66281-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 66281-1-Ig

  Western blot of YY1 in rat (C6 and ROS1728) and mouse(NIH3T3 and Raw264.7) cell lines with 66281-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66281-1-Ig

  WB result of YY1 antibody (66281-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YY1 transfected MCF-7 cells.

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  WB analysis using 66281-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66281-1-Ig (YY1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66281-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66281-1-Ig (YY1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66281-1-Ig

  Western blot of Hela cells with mouse anti-YY1 monoclonal antibody (66281-1-Ig) at various dilutions incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 66281-1-Ig

  COS-7 cells were subjected to SDS PAGE followed by western blot with 66281-1-Ig (YY1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 66281-1-Ig

  IP Result of anti-YY1 (IP:66281-1-Ig, 5ug; Detection:66281-1-Ig 1:1000) with NIH/3T3 cells lysate 4000ug.

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  IHC staining of human breast cancer using 66281-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66281-1-Ig (YY1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66281-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66281-1-Ig (YY1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66281-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66281-1-Ig (YY1 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66281-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66281-1-Ig(YY1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66281-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66281-1-Ig(YY1 antibody) at dilution of 1:800 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66281-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66281-1-Ig(YY1 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: C6 cells, A431 cells, MCF-7 cells, NIH/3T3 cells, HeLa cells, COS-7 cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

66281-1-Ig targets YY1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: YY1 fusion protein Ag17732
• Full Name: YY1 transcription factor
• Calculated molecular weight: 414 aa, 45 kDa
• Observed molecular weight: 65-70 kDa
• GenBank accession number: BC037308
• Gene symbol: YY1
• Gene ID (NCBI): 7528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.

Publications

Species	Application	Title
human	WB,IHC	Nat Commun; The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer.; Authors - Weimei Tang
	WB	Mol Cancer; CircPSMC3 suppresses the proliferation and metastasis of gastric cancer by acting as a competitive endogenous RNA through sponging miR-296-5p.; Authors - Dawei Rong
human	WB	J Cell Mol Med; FAM3C-YY1 axis is essential for TGFβ-promoted proliferation and migration of human breast cancer MDA-MB-231 cells via the activation of HSF1.; Authors - Weili Yang
human	WB	Med Oncol; RNF144A exerts tumor suppressor function in breast cancer through targeting YY1 for proteasomal degradation to downregulate GMFG expression.; Authors - Yin-Ling Zhang
human	WB,IHC	Prostate; hnRNPM, a potential mediator of YY1 in promoting the epithelial-mesenchymal transition of prostate cancer cells.; Authors - Tong Yang
		Environ Toxicol; Transcription factor YY1 enhances the stemness of lung cancer cells by stabilizing hypoxia factor HIF-1α under a hypoxic microenvironment.; Authors - Xianping Huang

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