YY1 Monoclonal antibody
YY1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, monkey
Applications
WB, IP, IHC, IF, CoIP, ChIP, ELISA
Conjugate
Unconjugated
CloneNo.
2E11C5
Cat no : 66281-1-Ig
Synonyms
"YY1 Antibodies" Comparison
View side-by-side comparison of YY1 antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
Positive WB detected in | C6 cells, A431 cells, MCF-7 cells, NIH/3T3 cells, HeLa cells, COS-7 cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells |
Positive IP detected in | NIH/3T3 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 15 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
CoIP | See 1 publications below |
ChIP | See 1 publications below |
Product Information
66281-1-Ig targets YY1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
Tested Reactivity | human, mouse, rat, monkey |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | YY1 fusion protein Ag17732 |
Full Name | YY1 transcription factor |
Calculated molecular weight | 414 aa, 45 kDa |
Observed molecular weight | 65-70 kDa |
GenBank accession number | BC037308 |
Gene symbol | YY1 |
Gene ID (NCBI) | 7528 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for YY1 antibody 66281-1-Ig | Download protocol |
IHC protocol for YY1 antibody 66281-1-Ig | Download protocol |
IF protocol for YY1 antibody 66281-1-Ig | Download protocol |
IP protocol for YY1 antibody 66281-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer. | ||
Mol Cancer CircPSMC3 suppresses the proliferation and metastasis of gastric cancer by acting as a competitive endogenous RNA through sponging miR-296-5p. | ||
J Cell Mol Med FAM3C-YY1 axis is essential for TGFβ-promoted proliferation and migration of human breast cancer MDA-MB-231 cells via the activation of HSF1. | ||
Med Oncol RNF144A exerts tumor suppressor function in breast cancer through targeting YY1 for proteasomal degradation to downregulate GMFG expression. | ||
Prostate hnRNPM, a potential mediator of YY1 in promoting the epithelial-mesenchymal transition of prostate cancer cells. | ||
Environ Toxicol Transcription factor YY1 enhances the stemness of lung cancer cells by stabilizing hypoxia factor HIF-1α under a hypoxic microenvironment. |