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WB analysis of Jurkat using 15592-1-AP
WB result of ZAP70 antibody (15592-1-AP, 1:20000) with si-control and si-ZAP70 transfected Jurkat cells.
WB analysis using 15592-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15592-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 15592-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 15592-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 15592-1-AP
IP result of anti-ZAP70 (IP:15592-1-AP, 4ug; Detection:15592-1-AP 1:1000) with Jurkat cells lysate 3200ug.
IHC staining of human tonsillitis using 15592-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human tonsillitis using 15592-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lymphoma using 15592-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lymphoma using 15592-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 15592-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 15592-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 15592-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 15592-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of RAW 264.7 using 15592-1-AP
Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using ZAP70 antibody (15592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

ZAP70 Polyclonal antibody

Name: ZAP70 Polyclonal antibody
Brand: Proteintech
SKU: 15592-1-AP
Rating: 5.00 (2 reviews)

ZAP70 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15592-1-AP

Publications(1)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

70 kDa zeta associated protein, 70 kDa zeta-chain associated protein, EC:2.7.10.2, SRK, STD

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, A375 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human tonsillitis tissue, human kidney tissue, human lymphoma tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:20000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

15592-1-AP targets ZAP70 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7943 Product name: Recombinant human ZAP70 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 149-493 aa of BC039039 Sequence: STLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA Predict reactive species
Full Name	zeta-chain (TCR) associated protein kinase 70kDa
Calculated Molecular Weight	70 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC039039
Gene Symbol	ZAP70
Gene ID (NCBI)	7535
ENSEMBL Gene ID	ENSG00000115085
RRID	AB_2218654
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P43403
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

Protocols

Product Specific Protocols
IF protocol for ZAP70 antibody 15592-1-AP	Download protocol
IHC protocol for ZAP70 antibody 15592-1-AP	Download protocol
IP protocol for ZAP70 antibody 15592-1-AP	Download protocol
WB protocol for ZAP70 antibody 15592-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Immunol Methods An optimized non-T cell transfection system based on HEK293FT cells for CD3ζ phosphorylation and ubiquitination Authors - Jiaqi Zheng View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Titouan (Verified Customer) (01-31-2025)	Works very well Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Jurkat ZAP70 Antibody Western Blot validation (1:1000 dilution) in Jurkat (Cat no:15592-1-AP)
FH hala (Verified Customer) (01-27-2023)	works very good Applications: Western Blot Primary Antibody Dilution: 1/10000 Cell Tissue Type: hepG2 ZAP70 Antibody Western Blot validation (1/10000 dilution) in hepG2 (Cat no:15592-1-AP)