Tested Applications
| Positive WB detected in | Jurkat cells, A375 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue, human kidney tissue, human lymphoma tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15592-1-AP targets ZAP70 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7943 Product name: Recombinant human ZAP70 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 149-493 aa of BC039039 Sequence: STLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA Predict reactive species |
| Full Name | zeta-chain (TCR) associated protein kinase 70kDa |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC039039 |
| Gene Symbol | ZAP70 |
| Gene ID (NCBI) | 7535 |
| ENSEMBL Gene ID | ENSG00000115085 |
| RRID | AB_2218654 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43403 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZAP70 antibody 15592-1-AP | Download protocol |
| IHC protocol for ZAP70 antibody 15592-1-AP | Download protocol |
| IP protocol for ZAP70 antibody 15592-1-AP | Download protocol |
| WB protocol for ZAP70 antibody 15592-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZAP70 Polyclonal antibody (Cat# 15592-1-AP) has 2 total citations. One appeared in Nature Immunology (2026), studying VSIG4-CD5-mediated T cell responses and tumor evasion in mice. The other was published in Journal of Immunological Methods (2024), investigating CD3ζ phosphorylation and ubiquitination in human HEK293FT cells. Both citations used Western blot. The associated research fields are T cell receptor signaling, tumor immunology, and immunological methods.
| Species | Application | Title |
|---|---|---|
Nat Immunol Kupffer cell calibration of T cell responses via VSIG4-CD5 interaction promotes tumor evasion. | ||
J Immunol Methods An optimized non-T cell transfection system based on HEK293FT cells for CD3ζ phosphorylation and ubiquitination |
Reviews
What customers say
All three reviews rated this antibody 5 out of 5 stars. Users consistently reported excellent performance in Western Blot, with one also confirming successful use in Immunohistochemistry. Tested cell lines included Jurkat (immune cell line) and HepG2, with working dilutions ranging from 1:1,000 to 1:10,000. Reviewers described the antibody as "very good" and noted it "works very well," indicating reliable detection and strong signal across different cell types and dilutions. Overall, the feedback reflects high satisfaction with specificity and performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hadi (Verified Customer) (03-20-2026) | Very good, works very well in Western blot of Jurkat cell extract
|
Titouan (Verified Customer) (01-31-2025) | Works very well
 |
hala (Verified Customer) (01-27-2023) | works very good
 |
