ZBTB11 Polyclonal antibody

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  WB analysis of mouse liver using 25215-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25215-1-AP (ZBTB11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 25215-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25215-1-AP (ZBTB11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human urothelial carcinoma using 25215-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 25215-1-AP (ZBTB11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 25215-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 25215-1-AP (ZBTB11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

ZBTB11 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25215-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Zinc finger and BTB domain-containing protein 11, ZNF U69274

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Tested Applications

• Positive WB detected in: mouse liver tissue, PC-3 cells
• Positive IHC detected in: human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

25215-1-AP targets ZBTB11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17000

  Product name: Recombinant human ZBTB11 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 704-1053 aa of BC111700

  Sequence: QKQFQCELCVKSFVTKRSLQEHMSIHTGESKYLCSVCGKSFHRGSGLSKHFKKHQPKPEVRGYHCTQCEKSFFEARDLRQHMNKHLGVKPFQCQFCDKCYSWKKDWYSHVKSHSVTEPYRCNICGKEFYEKALFRRHVKKATHGKKGRAKQNLERVCEKCGRKFTQLREYRRHMNNHEGVKPFECLTCGVAWADARSLKRHVRTHTGERPYVCPVCSEAYIDARTLRKHMTKFHRDYVPCKIMLEKDTLQFHNQGTQVAHAVSILTAGMQEQESSGPQELETVVVTGETMEALEAVAATEEYPSVSTLSDQSIMQVVNYVLAQQQGQKLSEVAEAIQTVKVEVAHISGGE

• Full Name: zinc finger and BTB domain containing 11
• Calculated Molecular Weight: 1053 aa, 119 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC111700
• Gene Symbol: ZBTB11
• Gene ID (NCBI): 27107
• RRID: AB_2879964
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95625
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger and BTB domain containing 11 (ZBTB11), also named as ZNF U69274, is a 1053 amino acid protein, which contains one BTB domain and twelve C2H2-type zinc fingers. ZBTB11 localizes in the nucleus and may be involved in transcriptional regulation. The molecular weight of ZBTB11 is 119 kDa, but the phosphorylated ZBTB11 is a 135-140 kDa protein.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Acta Pharmacol Sin; DUBR suppresses migration and invasion of human lung adenocarcinoma cells via ZBTB11-mediated inhibition of oxidative phosphorylation.; Authors - Wei Nie ; KD Validated