ZBTB4 Polyclonal antibody

• 

  WB analysis using 17611-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17611-1-AP (ZBTB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ZBTB4 Polyclonal Antibody for WB, ELISA

Cat No. 17611-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

KAISO L1, KAISO-L1, KAISO-like zinc finger protein 1, ZBTB 4, Zinc finger and BTB domain-containing protein 4

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Tested Applications

• Positive WB detected in: DU 145 cells, MCF-7 cells, MDA-MB-231 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17611-1-AP targets ZBTB4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11774

  Product name: Recombinant human ZBTB4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-353 aa of BC043352

  Sequence: MPPPAEVTDPSHAPAVLRQLNEQRLRGLFCDVTLIAGDTKFPAHRSVLAASSPFFREALLTSAPLPLPPATGGAAPNPATTTAASSSSSSSSSSSSSSSSASSSSSSSSSSPPPASPPASSPPRVLELPGVPAAAFSDVLNFIYSARLALPGGGGDGAAVAEIGALGRRLGISRLQGLGEGGDAWVPSTPAPMATSQPEEDSFGPGPRPAGEWEGDRAEAQAPDLQCSLPRRPLPCPQCGKSFIHPKRLQTHEAQCRRGASTRGSTGLGAGGAGPGGPAGVDASALPPPVGFRGGPEHVVKVVGGHVLYVCAACERSYVTLSSLKRHSNVHSWRRKYPCRYCEKVFALAEYRT

• Full Name: zinc finger and BTB domain containing 4
• Calculated Molecular Weight: 1013 aa, 105 kDa
• Observed Molecular Weight: 105-140 kDa
• GenBank Accession Number: BC043352
• Gene Symbol: ZBTB4
• Gene ID (NCBI): 57659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P1Z0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZBTB4 (zinc finger and BTB domain containing 4), also known as KAISO-L1 (KAISO-like zinc finger protein 1), is a 1,013 amino acid nuclear protein that is involved in transcriptional regulation. ZBTB4 contains one BTB (POZ) domain, six C2H2-type zinc fingers and is phosphorylated and downregulated by HIPK2. The gene encoding ZBTB4 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Clin Transl Med; Glioma glycolipid metabolism: MSI2-SNORD12B-FIP1L1-ZBTB4 feedback loop as a potential treatment target.; Authors - Weiwei Dong