mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21324-1-AP
WB result of ZDHHC5 antibody (21324-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZDHHC5 transfected HeLa cells.
WB result of ZDHHC5 antibody (21324-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZDHHC5 transfected HeLa cells.
WB analysis of HEK-293 using 21324-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 21324-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 21324-1-AP
IP result of anti-ZDHHC5 (IP:21324-1-AP, 4ug; Detection:21324-1-AP 1:500) with HEK-293 cells lysate 1680 ug.
Immunofluorescent analysis of Hela cells, using ZDHHC5 antibody 21324-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HeLa using 21324-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ZDHHC5 antibody (21324-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ZDHHC5 antibody (21324-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 21324-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ZDHHC5 (21324-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ZDHHC5 (21324-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues.
AKAP150 and its Palmitoylation Contributed to Pain Hypersensitivity Via Facilitating Synaptic Incorporation of GluA1-Containing AMPA Receptor in Spinal Dorsal Horn.
Palmitoylation enhances the stability of porcine epidemic diarrhea virus spike protein by antagonizing its degradation via chaperone-mediated autophagy to facilitate viral proliferation
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21324-1-AP
WB result of ZDHHC5 antibody (21324-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZDHHC5 transfected HeLa cells.
WB analysis of HEK-293 using 21324-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 21324-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP Figures
IP experiment of HEK-293 using 21324-1-AP
IP result of anti-ZDHHC5 (IP:21324-1-AP, 4ug; Detection:21324-1-AP 1:500) with HEK-293 cells lysate 1680 ug.
IF/ICC Figures
IF Staining of HeLa using 21324-1-AP
Immunofluorescent analysis of Hela cells, using ZDHHC5 antibody 21324-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HeLa using 21324-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ZDHHC5 antibody (21324-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC (INTRA) Figures
FC experiment of HeLa using 21324-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ZDHHC5 (21324-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
ZDHHC5 Polyclonal antibody
Catalog Number
21324-1-AP
Citations
21
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IF/ICC : 1:200-1:800 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IF/ICC, FC (Intra), IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
