ZFP36 Polyclonal antibody

ZFP36 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12737-1-AP

Synonyms

G0S24, GOS24, NUP475, Protein TIS11A, RNF162A, TIS11, TIS11A, Tristetraproline, TTP, Zfp 36, ZFP36, Zinc finger protein 36 homolog



Tested Applications

Positive WB detected inmouse lung tissue, A549 cells
Positive IHC detected inhuman breast cancer tissue, human bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12737-1-AP targets ZFP36 in WB, RIP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZFP36 fusion protein Ag3461
Full Name zinc finger protein 36, C3H type, homolog (mouse)
Calculated molecular weight 326 aa, 34 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC009693
Gene symbol ZFP36
Gene ID (NCBI) 7538
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The expression of many cytokines is regulated post-transcriptionally by factors that modulate mRNA transport, translation, and stability. Much of this regulation occurs by the binding and stabilizing, or destabilizing, of cytokine mRNAs by proteins that recognize adenosine and uridine-rich elements (AREs) in untranslated regions of target transcripts. Zfp36 is a mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. It was demonstrated to physically interact with the p65 subunit of nuclear factor-κB leading to decreased nuclear import and diminished transcriptional activation mediated by nuclear factor-κB. It acted by specifically binding ARE-containing mRNAs and promoting their degradation, and has a crucial role in the post-transcriptional regulation of tumor necrosis factor (TNF). The calcualted molecular weight of ZFP36 is 34 kDa, but modified ZFP36 is about 40-45 kDa.

Protocols

Product Specific Protocols
WB protocol for ZFP36 antibody 12737-1-APDownload protocol
IHC protocol for ZFP36 antibody 12737-1-APDownload protocol
IF protocol for ZFP36 antibody 12737-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

FASEB J

Tristetraprolin destabilizes NOX2 mRNA and protects dopaminergic neurons from oxidative damage in Parkinson's disease.

Authors - Xiang Sun
humanWB

Oncotarget

Inhibition of COX2 enhances the chemosensitivity of dichloroacetate in cervical cancer cells.

Authors - Bo Li
humanWB

Sci Rep

The Fragment HMGA2-sh-3p20 from HMGA2 mRNA 3'UTR Promotes the Growth of Hepatoma Cells by Upregulating HMGA2.

Authors - Yuan Wang

Proc Natl Acad Sci U S A

Brf1 posttranscriptionally regulates pluripotency and differentiation responses downstream of Erk MAP kinase.

Authors - Frederick E Tan
mouseWB,RIP

Dev Cell

N6-Adenosine Methylation of Socs1 mRNA Is Required to Sustain the Negative Feedback Control of Macrophage Activation.

Authors - Jie Du