ZNF154 Polyclonal antibody

ZNF154 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22161-1-AP

Synonyms

KIAA2003, pHZ 92, zinc finger protein 154, ZNF154

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Validation Data Gallery

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  WB analysis of COS-7 using 22161-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22161-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22161-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 22161-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: COS-7 cells, A549 cells, HEK-293 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22161-1-AP targets ZNF154 in WB, ELISA applications and shows reactivity with human, monkey samples.

• Tested Reactivity: human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF154 fusion protein Ag17812
• Full Name: zinc finger protein 154
• Calculated Molecular Weight: 437 aa, 50 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC152404
• Gene Symbol: ZNF154
• Gene ID (NCBI): 7710
• RRID: AB_2879012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sites that display recurrent, aberrant DNA methylation in cancer represent potential biomarkers for screening and diagnostics. Previously, The hypermethylation at the ZNF154 CpG island in 15 solid epithelial tumor types from 13 different organs. The magnitude and reproducibility of the ZNF154 hypermethylation signal across five solid tumor types reinforces the potential of this site as a biomarker for circulating tumor DNA (ctDNA)(PMID: 26857064 ). The calculated molecular weight of ZNF154 is 50 kDa, but the modified ZNF154 protein is about 55-60 kDa.