ZNF154 Polyclonal antibody, PBS Only

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  WB analysis of COS-7 using 22161-1-AP (same clone as 22161-1-PBS)

  COS-7 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22161-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 22161-1-AP (same clone as 22161-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22161-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 22161-1-AP (same clone as 22161-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22161-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 22161-1-AP (same clone as 22161-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22161-1-PBS in a different storage buffer formulation.

ZNF154 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 22161-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, Indirect ELISA

Synonyms

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Product Information

22161-1-PBS targets ZNF154 in WB, Indirect ELISA applications and shows reactivity with human, monkey samples.

• Tested Reactivity: human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF154 fusion protein Ag17812
• Full Name: zinc finger protein 154
• Calculated Molecular Weight: 437 aa, 50 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC152404
• Gene Symbol: ZNF154
• Gene ID (NCBI): 7710
• RRID: AB_2879012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13106
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Sites that display recurrent, aberrant DNA methylation in cancer represent potential biomarkers for screening and diagnostics. Previously, The hypermethylation at the ZNF154 CpG island in 15 solid epithelial tumor types from 13 different organs. The magnitude and reproducibility of the ZNF154 hypermethylation signal across five solid tumor types reinforces the potential of this site as a biomarker for circulating tumor DNA (ctDNA)(PMID: 26857064 ). The calculated molecular weight of ZNF154 is 50 kDa, but the modified ZNF154 protein is about 55-60 kDa.