ZNF280B Polyclonal antibody

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  WB analysis using 32519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32519-1-AP (ZNF280B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 32519-1-AP

  IP result of anti-ZNF280B (IP:32519-1-AP, 4ug; Detection:32519-1-AP 1:1500) with K-562 cells lysate 2440 ug.

ZNF280B Polyclonal Antibody for WB, IP, ELISA

Cat No. 32519-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

5'OY11.1, SUHW2, Suppressor of hairy wing homolog 2, Zinc finger protein 279, Zinc finger protein 280B

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Tested Applications

• Positive WB detected in: K-562 cells, U2OS cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32519-1-AP targets ZNF280B in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38544

  Product name: Recombinant human ZNF280B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 75-268 aa of NM_080764.3

  Sequence: YDHLRKDTARKLQPKSHETVTSEAVTVLPASQLESRSTDSPIIIEPLSKPDYRNSSPQVVPNNSSELPSPLITFTDSLHHPVSTALSVGGINESPRVSKQLSTFEVNSINPKRAKLRDGIIEGNSSASFPSDTFHTMNTQQSTPSNNVHTSLSHVQNGAPFPAAFPKDNIHFKPINTNLDRANELAKTDILSLT

• Full Name: zinc finger protein 280B
• Calculated Molecular Weight: 62kDa，543aa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_080764.3
• Gene Symbol: ZNF280B
• Gene ID (NCBI): 140883
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q86YH2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF280B belongs to the family of zinc finger proteins, a transcription factor that is mainly involved in the regulation of gene expression. In prostate cancer cells, ZNF280B induces p53 nuclear export, leading to subsequent proteasomal degradation. Therefore, ZNF280B has pro-growth and pro-survival functions in prostate cancer cells. In addition to this, ZNF280B has a promotional role in gastric and lung cancer development, and its potential as a tumor therapeutic target can be explored in the future.