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Tested Applications
| Positive WB detected in | SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21365-1-AP targets ZNF543 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15830 Product name: Recombinant human ZNF543 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 132-225 aa of BC101015 Sequence: EVHLKIGIGPQRGKLLEKMSSERDGLGSDDGVCTKITQKQVSTEGDLYECDSHGPVTDALIREEKNSYKCEECGKVFKKNALLVQHERIHTQVK Predict reactive species |
| Full Name | zinc finger protein 543 |
| Calculated Molecular Weight | 600 aa, 68 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC101015 |
| Gene Symbol | ZNF543 |
| Gene ID (NCBI) | 125919 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08ER8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZNF543 is a member of the Kruppel-associated box (KRAB) containing zinc finger proteins family, which function as transcription factors. ZNF543 has a wide range of physiological functions in a variety of cellular processes, which including apoptosis, cell proliferation and differentiation (PMID: 35467255, PMID: 34975305).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF543 antibody 21365-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
