ZNF573 Polyclonal antibody

ZNF573 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24755-1-AP

Synonyms

zinc finger protein 573, ZNF573

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Validation Data Gallery

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  IHC staining of human liver using 24755-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24755-1-AP (ZNF573 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 24755-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24755-1-AP (ZNF573 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 24755-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24755-1-AP (ZNF573 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 24755-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24755-1-AP (ZNF573 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 24755-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using ZNF573 antibody (24755-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human liver tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: U-251 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24755-1-AP targets ZNF573 in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF573 fusion protein Ag20475
• Full Name: zinc finger protein 573
• Calculated Molecular Weight: 645 aa, 76 kDa
• GenBank Accession Number: BC042170
• Gene Symbol: ZNF573
• Gene ID (NCBI): 126231
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF573 belongs to the krueppel C2H2-type zinc-finger protein family. ZNF573 is involved in a broad range of biological functions including apoptosis, protein structure, DNA recognition, and RNA transcription, the structural variation in ZNF573 protein may result in the abnormal growth and development of human lens, further leading to the occurrence of cataracts.