Tested Applications
| Positive IP detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33033-1-AP targets ZNF589 in IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36351 Product name: Recombinant human ZNF589 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 76-250 aa of BC166625 Sequence: ESKPEVHTCPSCPLAFGSQQFLSQDELHNHPIPGFHAGNQLHPGNPCPEDQPQSQHPSDKNHRGAEAEDQRVEGGVRPLFWSTNERGALVGFSSLFQRPPISSWGGNRILEIQLSPAQNASSEEVDRISKRAETPGFGAVTFGECALAFNQKSNLFRQKAVTAEKSSDKRQSQVC Predict reactive species |
| Full Name | zinc finger protein 589 |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC166625 |
| Gene Symbol | ZNF589 |
| Gene ID (NCBI) | 51385 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q86UQ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZNF589(Zinc Finger Protein 589) is a protein coding gene located at 19q13.43 in human genome, belonging to C2H2 zinc finger protein family. Most members of this family appear in the form of "KRAB-ZNF", that is, the N-terminal has a KRAB(Krüppel-associated box) transcription inhibitory domain, and the C-terminal is a DNA binding domain composed of multiple C2H2 zinc fingers connected in series. ZNF589 also follows this classic structure: KRAB-A/B box at N end, middle linker, and 11 continuous C2H2 zinc fingers at C end. Because KRAB domain can recruit KAP1/TRIM28 and other co-inhibitory complexes, ZNF589 is generally regarded as a potential transcription inhibitor. GTEx data showed that the expression of ZNF589 was low to moderate in almost all adult tissues, with the highest expression in testis, ovary, thyroid, spleen, peripheral blood leukocytes and embryonic stem cells (hESC).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ZNF589 antibody 33033-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
