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ZO-1 Polyclonal antibody

ZO-1 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine, mouse, rat, hamster and More (3)

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 21773-1-AP

Synonyms

Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, ZO-1, Zona occludens protein 1, Zonula occludens protein 1



Tested Applications

Positive WB detected inA549 cells, A431 cells, COLO 320 cells, HEK-293 cells, HT-29 cells, HUVEC cells, HepG2 cells, MCF-7 cells, Caco-2 cells, HeLa cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHEK-293 cells
Positive IF detected inHUVEC cells, MCF-7 cells, human breast cancer tissue, MDCK cells, mouse colon tissue, mouse kidney tissue
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21773-1-AP is ZO-1 Fusion Protein expressed in E. coli.

Tested Reactivity human, canine, mouse, rat, hamster
Cited Reactivityhuman, mouse, rat, bovine, canine, chicken, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZO-1 fusion protein Ag16454
Full Name tight junction protein 1 (zona occludens 1)
Calculated molecular weight 1748 aa, 195 kDa
Observed molecular weight 230 kDa
GenBank accession numberBC111712
Gene symbol TJP1
Gene ID (NCBI) 7082
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the function of ZO-1?

Zona Occludens 1 (ZO-1) is a tight junction (TJ) protein found in complexes at cell-cell contacts. The role of ZO-1 is to recruit other TJ proteins.1,2 The resulting TJ complexes regulate paracellular flow, contribute to apical-basal polarity, and are part of signaling pathways for proliferation and differentiation.3 This protein is useful for highlighting cell-cell contacts both in cultured cells and in tissue samples, outlining cell membranes and specific structures.

 

Where is ZO-1 expressed?

The protein is localized to the cytoplasmic membrane in most types of cells, particularly where a barrier function is essential. It is common in endothelial cells,4 which line the internal surface of blood and lymph vessels, and in epithelial cells,5 which form the outer barrier of organs.

ZO-1 is expressed in many tissues including the intestine, kidney, liver, and skeletal muscle cells.

 

What proteins does ZO-1 interact with?

The molecular weight of ZO-1 is 220kDa6 and it contains multiple distinct protein domains that allow bind to other junctional proteins at the cytoplasmic membrane. The N-terminal of ZO-1 can dimerize with other ZO proteins and bind directly to other TJ proteins including claudins, connexions, and JAMs,7-10 which allows it to assemble TJ complexes. The C-terminal can interact with actin and cortactin, anchoring the TJ complexes to the cytoskeleton.8,11 This suggests that ZO-1 forms a link between the outer cell-cell contacts and the inner actin.

 

1.     McNeil, E., Capaldo, C. T. & Macara, I. G. Zonula occludens-1 function in the assembly of tight junctions in Madin-Darby canine kidney epithelial cells. Mol. Biol. Cell 17, 1922-32 (2006).

2.     Kratzer, I. et al. Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier. Histochem. Cell Biol. (2012). doi:10.1007/s00418-012-1001-9

3.     Guillemot, L., Paschoud, S., Pulimeno, P., Foglia, A. & Citi, S. The cytoplasmic plaque of tight junctions: A scaffolding and signalling center. Biochim. Biophys. Acta - Biomembr. 1778, 601-613 (2008).

4.     Tornavaca, O. et al. ZO-1 controls endothelial adherens junctions, cell-cell tension, angiogenesis, and barrier formation. J. Cell Biol. 208, 821-38 (2015).

5.     Umeda, K. et al. Establishment and characterization of cultured epithelial cells lacking expression of ZO-1. J. Biol. Chem. 279, 44785-94 (2004).

6.     Stevenson, B. R. Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia. J. Cell Biol. 103, 755-766 (1986).

7.     Itoh, M. et al. Direct Binding of Three Tight Junction-Associated Maguks, Zo-1, Zo-2, and Zo-3, with the Cooh Termini of Claudins. J. Cell Biol. 147, 1351-1363 (1999).

8.     Fanning, A. S., Jameson, B. J., Jesaitis, L. A. & Anderson, J. M. The Tight Junction Protein ZO-1 Establishes a Link between the Transmembrane Protein Occludin and the Actin Cytoskeleton. J. Biol. Chem. 273, 29745-29753 (1998).

9.     Kausalya, P. J., Reichert, M. & Hunziker, W. Connexin45 directly binds to ZO-1 and localizes to the tight junction region in epithelial MDCK cells. FEBS Lett. 505, 92-96 (2001).

10. Itoh, M. et al. Junctional adhesion molecule (JAM) binds to PAR-3. J. Cell Biol. 154, 491-498 (2001).

11. Itoh, M., Nagafuchi, A., Moroi, S. & Tsukita, S. Involvement of ZO-1 in Cadherin-based Cell Adhesion through Its Direct Binding to α Catenin and Actin Filaments. J. Cell Biol. 138, 181-192 (1997).  

Protocols

Product Specific Protocols
WB protocol for ZO-1 antibody 21773-1-APDownload protocol
IF protocol for ZO-1 antibody 21773-1-APDownload protocol
IP protocol for ZO-1 antibody 21773-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.

Authors - Xiaoyu Yu
mouseIF

EMBO J

Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.

Authors - Rongpan Bai
mouseIF

Biomaterials

A self-driven bioinspired nanovehicle by leukocyte membrane-hitchhiking for early detection and treatment of atherosclerosis.

Authors - Guanghao Wu
humanIHC

Biomaterials

Oral delivery of decanoic acid conjugated plant protein shell incorporating hybrid nanosystem leverage intestinal absorption of polyphenols.

Authors - Ruimin Miao
humanIF

Biomaterials

Magnetically-propelled fecal surrogates for modeling the impact of solid-induced shear forces on primary colonic epithelial cells.

Authors - Samuel S Hinman
mouseWB

Biomaterials

MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.

Authors - Mingming Song

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xie (Verified Customer) (10-26-2021)

Efficient Labelling from proteintech ZO1 Antibody

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:150
  • Cell Tissue Type: CaCo-2 cell line
ZO-1 Antibody Immunofluorescence validation (1:150 dilution) in CaCo-2 cell line (Cat no:21773-1-AP)
FH

Samuel (Verified Customer) (08-06-2021)

Clean and strong band at 220-230 kDa in WB (reducing and denaturing conditions). I used the antibody at a 1:2500 dilution in 5% milk, incubation overnight at 4 degrees Celsius. 30ug loaded per sample.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2500
  • Cell Tissue Type: SH-SY5Y, HEPG2, HEK293. 30ug each
ZO-1 Antibody Western Blot validation (1/2500 dilution) in SH-SY5Y, HEPG2, HEK293. 30ug each (Cat no:21773-1-AP)