• Featured Product
  • KD/KO Validated

Zyxin Polyclonal antibody

Zyxin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10330-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

ZYX, Zyxin-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10330-1-AP targets Zyxin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0419

Product name: Recombinant human ZYX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 278-572 aa of BC008743

Sequence: SKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYKCEDCGKPLSIEADDNGCFPLDGHVLCRKCHTARAQT

Predict reactive species
Full Name zyxin
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 78 kDa
GenBank Accession NumberBC008743
Gene Symbol Zyxin
Gene ID (NCBI) 7791
RRIDAB_2221279
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15942
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin (ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.

Protocols

Product Specific Protocols
WB protocol for Zyxin antibody 10330-1-APDownload protocol
IHC protocol for Zyxin antibody 10330-1-APDownload protocol
IF protocol for Zyxin antibody 10330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Cell Biol

The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.

Authors - Natalia Schiefermeier
mouse,humanWB,IF

Cell Death Dis

Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression

Authors - Rong Yan
  • KD Validated
rabbitIF

Cell Prolif

Zyxin-involved actin regulation is essential in the maintenance of vinculin focal adhesion and chondrocyte differentiation status.

Authors - Gaoming Li
  • KD Validated
humanWB,IP,IF

J Biol Chem

O-GlcNAc transferase promotes the nuclear localization of the focal adhesion-associated protein Zyxin to regulate UV-induced cell death.

Authors - Yuhang Zhao
mouseWB

Am J Physiol Gastrointest Liver Physiol

Targeted disruption of the Lasp-1 gene is linked to increases in histamine-stimulated gastric HCl secretion.

Authors - Chew Catherine S CS
mouseWB,IF

Physiol Genomics

Lasp1 gene disruption is linked to enhanced cell migration and tumor formation.

Authors - Zhang Han H

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Alex (Verified Customer) (08-20-2025)

Excellent staining of adhesions and actin filament tips. Consistent with literature-reported fluorescent protein constructs. In image: A - Paxillin (from another manufacturer) B - Zyxin (10330-1-AP. 1:200) C - Phalloidin (from another manufacturer) D - Merged (G = Paxillin; R = Zyxin; gray = phalloidin)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: neural
Zyxin Antibody Immunofluorescence validation (1:200 dilution) in neural (Cat no:10330-1-AP)
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