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  • KD/KO Validated

Zyxin Polyclonal antibody

Zyxin Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 10330-1-AP

Synonyms

ESP 2, HED 2, ZYX, zyxin, Zyxin 2



Tested Applications

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10330-1-AP targets Zyxin in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Zyxin fusion protein Ag0419
Full Name zyxin
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 78 kDa
GenBank Accession NumberBC008743
Gene Symbol ZYX
Gene ID (NCBI) 7791
RRIDAB_2221279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin (ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.

Protocols

Product Specific Protocols
WB protocol for Zyxin antibody 10330-1-APDownload protocol
IF protocol for Zyxin antibody 10330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Cell Biol

The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.

Authors - Natalia Schiefermeier
human,mouseWB,IF

Cell Death Dis

Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression

Authors - Rong Yan
  • KD Validated
rabbitIF

Cell Prolif

Zyxin-involved actin regulation is essential in the maintenance of vinculin focal adhesion and chondrocyte differentiation status.

Authors - Gaoming Li
  • KD Validated
humanWB,IF,IP

J Biol Chem

O-GlcNAc transferase promotes the nuclear localization of the focal adhesion-associated protein Zyxin to regulate UV-induced cell death.

Authors - Yuhang Zhao
mouseWB

Am J Physiol Gastrointest Liver Physiol

Targeted disruption of the Lasp-1 gene is linked to increases in histamine-stimulated gastric HCl secretion.

Authors - Chew Catherine S CS
mouseWB,IF

Physiol Genomics

Lasp1 gene disruption is linked to enhanced cell migration and tumor formation.

Authors - Zhang Han H