|Positive WB detected in||A549 cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Sample-dependent, check data in validation data gallery|
10330-1-AP targets Zyxin in WB, IF,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||mouse, rabbit|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Zyxin fusion protein Ag0419|
|Calculated molecular weight||80 kDa|
|Observed molecular weight||78 kDa|
|GenBank accession number||BC008743|
|Gene ID (NCBI)||7791|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin (ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.
Lasp1 gene disruption is linked to enhanced cell migration and tumor formation.
Am J Physiol Gastrointest Liver Physiol
Targeted disruption of the Lasp-1 gene is linked to increases in histamine-stimulated gastric HCl secretion.
Zyxin-involved actin regulation is essential in the maintenance of vinculin focal adhesion and chondrocyte differentiation status.
J Cell Biol
The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.