Dynamitin (1-86 AA) Polyclonal antibody

Dynamitin (1-86 AA) Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 10032-1-AP

Synonyms

DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, DYNAMITIN, p50 dynamitin, RBP50



Tested Applications

Positive WB detected inmouse lung tissue, human brain tissue, human heart tissue, human testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

10032-1-AP targets Dynamitin (1-86 AA) in WB,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Dynamitin (1-86 AA) fusion protein Ag0029
Full Name dynactin 2 (p50)
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberAY189155
Gene symbol DCTN2
Gene ID (NCBI) 10540
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the N terminal 86aa of full length p50 dynamitin.

Protocols

Product Specific Protocols
WB protocol for Dynamitin (1-86 AA) antibody 10032-1-APDownload protocol
Standard Protocols
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