Dynamitin (1-86 AA) Polyclonal antibody

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  WB analysis of mouse lung using 10032-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10032-1-AP (Dynamitin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10032-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10032-1-AP (Dynamitin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10032-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10032-1-AP (Dynamitin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10032-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10032-1-AP (Dynamitin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Dynamitin (1-86 AA) Polyclonal Antibody for WB, ELISA

Cat No. 10032-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, DYNAMITIN, p50 dynamitin, RBP50

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Tested Applications

• Positive WB detected in: mouse lung tissue, human brain tissue, human heart tissue, human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10032-1-AP targets Dynamitin (1-86 AA) in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Dynamitin (1-86 AA) fusion protein Ag0029
• Full Name: dynactin 2 (p50)
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: AY189155
• Gene Symbol: Dynamitin
• Gene ID (NCBI): 10540
• RRID: AB_2090611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13561
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the N terminal 86aa of full length p50 dynamitin.