GFP tag Polyclonal antibody

GFP tag Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

aequorea victoria, recombinant protein

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 50430-2-AP

Synonyms

CFP, eGFP, eYFP, GFP, GFP tag, YFP



Tested Applications

Positive WB detected inrecombinant protein, recombinant protein protein, Transfected HEK-293 cells
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

50430-2-AP targets GFP tag in WB, IP, IHC, IF, ELISA applications and shows reactivity with aequorea victoria, recombinant protein samples.

Tested Reactivity aequorea victoria, recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GFP tag fusion protein Ag2128
Full Name GFP tag
Calculated molecular weight 26 kDa
GenBank accession numberAVU73901
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.

Protocols

Product Specific Protocols
WB protocol for GFP tag antibody 50430-2-APDownload protocol
IF protocol for GFP tag antibody 50430-2-APDownload protocol
IP protocol for GFP tag antibody 50430-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Biochem Parasitol

Stable transfection of Eimeria necatrix through nucleofection of second generation merozoites.

Authors - Chunhui Duan
WB

Microb Cell Fact

Rational construction of genome-reduced and high-efficient industrial Streptomyces chassis based on multiple comparative genomic approaches.

Authors - Qing-Ting Bu

Cell Rep

The EF-Hand Protein CALML6 Suppresses Antiviral Innate Immunity by Impairing IRF3 Dimerization.

Authors - Ziyang Wang

J Biol Chem

Retractile lysyl-tRNA synthetase-AIMP2 assembly in the human multi-aminoacyl-tRNA synthetase complex.

Authors - Zhoufei Hei

Dev Comp Immunol

Molecular cloning and characterization of the pig MHC class Ⅰ-related MR1 gene.

Authors - Xingxing Xiao

J Virol

Dissecting the cell entry pathway of baculovirus by single particle tracking and quantitative electron microscopic analysis.

Authors - Fujun Qin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Paul (Verified Customer) (01-15-2020)

Works well for WB.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
FH

Laura (Verified Customer) (01-14-2020)

Good sensitivity for Western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
FH

Aamir (Verified Customer) (01-08-2020)

Works well for WB and IF

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: HEKs
FH

Erica (Verified Customer) (09-26-2019)

This antibody works very well with both western blots and IP. We previously used GFP antibody from another company and it didn't work well. We then switched to Proteinntech's and the signal was very strong! Highly recommend!

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: 1:2000 WB
  • Cell Tissue Type: HEK, MEF, HELA
FH

Mayur (Verified Customer) (04-30-2019)

The H4 cells were transfected with pcDNA only and pcDNA EGFP . The expression of the EGFP was measured using the GFP antibody from ProteinTech. Great antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5K
  • Cell Tissue Type: H4 cells
GFP tag Antibody Western Blot, validation (1:5K dilution) in H4 cells (Cat no:50430-2-AP)