GFP tag Polyclonal antibody
GFP tag Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
aequorea victoria, recombinant protein and More (1)
Applications
WB, RIP, IP-MS, IP, IHC, IF, CoIP, ChIP, ELISA
Conjugate
Unconjugated
433
Cat no : 50430-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | recombinant protein, Transfected HEK-293 cells |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Product Information
50430-2-AP targets GFP tag in WB, RIP, IP-MS, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with aequorea victoria, recombinant protein samples.
| Tested Reactivity | aequorea victoria, recombinant protein |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GFP tag fusion protein Ag2128 |
| Full Name | GFP tag |
| Calculated molecular weight | 26 kDa |
| GenBank accession number | U73901 |
| Gene symbol | |
| Gene ID (NCBI) | |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GFP tag antibody 50430-2-AP | Download protocol |
| IF protocol for GFP tag antibody 50430-2-AP | Download protocol |
| IP protocol for GFP tag antibody 50430-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gastroenterology PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis | ||
Nat Genet Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome. | ||
Nat Methods TALEN-mediated precise genome modification by homologous recombination in zebrafish. | ||
Immunity Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine. | ||
Cell Metab TNFAIP3 Interacting Protein 3 Overexpression Suppresses Nonalcoholic Steatohepatitis by Blocking TAK1 Activation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Stephen (Verified Customer) (08-02-2022) | HeLa cells transfected with plasmid expressing EGFP fixed with 4% PFA for 15 mins and immunostained with rabbit GFP antibody at 4 degrees incubated for 20h washed then incubated secondary alexa 594 and DAPI for 1 hour room temp imaged on wide field fluorescence microscope.
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FH A (Verified Customer) (01-04-2022) | Works for IP
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FH Rinalda (Verified Customer) (06-21-2021) | Great product
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FH Lana (Verified Customer) (12-22-2020) | SDS-PAGE: 15 ug/ul RIPA protein lysate, 4-12% Bis-Tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) for 2h at 80V, 4C.Blocking: SEA Block Blocking Buffer 1h, room T.Primary Ab: O/N incubation at 4C, 1:2500.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.Lines of WB image: 1 – protein ladder, 2 – HEK293 whole cell lysate, negative transfection, 3 – whole cell lysate of cells transfected with eGFP.
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FH Paul (Verified Customer) (01-15-2020) | Works well for WB.
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FH Laura (Verified Customer) (01-14-2020) | Good sensitivity for Western blot.
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FH Aamir (Verified Customer) (01-08-2020) | Works well for WB and IF
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FH Erica (Verified Customer) (09-26-2019) | This antibody works very well with both western blots and IP. We previously used GFP antibody from another company and it didn't work well. We then switched to Proteinntech's and the signal was very strong! Highly recommend!
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FH Mayur (Verified Customer) (04-30-2019) | The H4 cells were transfected with pcDNA only and pcDNA EGFP . The expression of the EGFP was measured using the GFP antibody from ProteinTech. Great antibody.
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