|Positive WB detected in||recombinant protein, recombinant protein protein, Transfected HEK-293 cells|
|Positive IP detected in||Transfected HEK-293 cells|
|Positive IF detected in||Transfected HEK-293 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
50430-2-AP targets GFP tag in WB, RIP, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with aequorea victoria, recombinant protein samples.
|Tested Reactivity||aequorea victoria, recombinant protein|
|Cited Reactivity||mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GFP tag fusion protein Ag2128|
|Full Name||GFP tag|
|Calculated molecular weight||26 kDa|
|GenBank accession number||U73901|
|Gene ID (NCBI)|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lana (Verified Customer) (12-22-2020)
SDS-PAGE: 15 ug/ul RIPA protein lysate, 4-12% Bis-Tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) for 2h at 80V, 4C.Blocking: SEA Block Blocking Buffer 1h, room T.Primary Ab: O/N incubation at 4C, 1:2500.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.Lines of WB image: 1 – protein ladder, 2 – HEK293 whole cell lysate, negative transfection, 3 – whole cell lysate of cells transfected with eGFP.
Paul (Verified Customer) (01-15-2020)
Works well for WB.
Laura (Verified Customer) (01-14-2020)
Good sensitivity for Western blot.
Aamir (Verified Customer) (01-08-2020)
Works well for WB and IF
Erica (Verified Customer) (09-26-2019)
This antibody works very well with both western blots and IP. We previously used GFP antibody from another company and it didn't work well. We then switched to Proteinntech's and the signal was very strong! Highly recommend!
Mayur (Verified Customer) (04-30-2019)
The H4 cells were transfected with pcDNA only and pcDNA EGFP . The expression of the EGFP was measured using the GFP antibody from ProteinTech. Great antibody.