GFP tag Antibody

GFP tag Monoclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E10H7

Cat no : 66002-1-Ig

Synonyms

CFP, eGFP, eYFP, GFP, GFP tag, YFP



Tested Applications

Positive WB detected inRecombinant Protein
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

66002-1-Ig targets GFP tag in WB, IP, IHC, IF, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GFP tag fusion protein Ag2128
Full Name GFP tag
Calculated molecular weight 26 kDa
GenBank accession numberAVU73901
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Green fluorescence protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) derived from the jellyfish Aequorea Victoria which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm). GFP, when exposed to light in the blue to ultraviolet spectrum, will show a bright green fluorescent light, making it a very useful tool in research.

What is the molecular weight of GFP?

26.9 kDa

How does GFP work?

GFP was first isolated from the jellyfish Aequorea Victoria, a source of bioluminescence, in the 1960s and in 2008 the Nobel Prize in Chemistry was awarded “for the discovery and development of the green fluorescent protein, GFP” to Osamu Shimomura and colleagues, who recognized its potential in research (PMID: 13911999). A short amino acid sequence within the protein acts as the chromophore, which absorbs UV light at 395 nm and emits green light at 509 nm. 

Why is GFP a useful reporter?

When GFP was sequenced in 1992 (PMID: 1347277) it allowed scientists to express it in other organisms using transgenic techniques. It does not require cofactors to work, is non-toxic to live cells, and is relatively small, making it ideal as a “tag” for other proteins, identifiable by shining a UV light and observing the green fluorescence. The tertiary folded structure of GFP forms a chromophore at the center of a barrel shape, which protects the fluorescence-emitting amino acid chain from solvents, meaning it can function in many environments (PMID 9759496).

What are the applications for GFP?

When expressed attached to another protein, GFP can be used as a reporter gene to measure expression levels or can easily be used in fluorescence microscopy. It has been used to highlight proteins in a variety of model organisms, including bacteria, zebrafish, and mice.



Protocols

Product Specific Protocols
WB protocol for GFP tag antibody 66002-1-IgDownload protocol
IF protocol for GFP tag antibody 66002-1-IgDownload protocol
IP protocol for GFP tag antibody 66002-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Virol

The Carboxyl Terminus of Tegument Protein pUL21 Contributes to Pseudorabies Virus Neuroinvasion.

Authors - Kai Yan
IF

Neuropharmacology

Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn.

Authors - Zi-Yang Zhang

Cell Rep

The EF-Hand Protein CALML6 Suppresses Antiviral Innate Immunity by Impairing IRF3 Dimerization.

Authors - Ziyang Wang

Front Genet

A Novel α-Galactosidase A Splicing Mutation Predisposes to Fabry Disease.

Authors - Ping Li

Mol Cell

CHP1 Regulates Compartmentalized Glycerolipid Synthesis by Activating GPAT4.

Authors - Xiphias Ge Zhu

Nucleic Acids Res

SCRE serves as a unique synaptonemal complex fastener and is essential for progression of meiosis prophase I in mice.

Authors - Hongbin Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jane (Verified Customer) (03-02-2020)

Adenovirus-GFP infected cardiomyocytes stained with GFP antibody, signal is strong and effective

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: cardiomyocyte culture
FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

  • Applications: Immunofluorescence,
  • Cell Tissue Type: HEART
FH

Jie (Verified Customer) (01-27-2020)

Worked for western blot with GFP-LC3 transfected cardiomyocytes

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: cardiomyocytes
FH

Paul (Verified Customer) (01-15-2020)

Works well for Westerns.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: HEK293T
FH

Laura (Verified Customer) (01-15-2020)

Good antibody for Western Blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293T
FH

Aamir (Verified Customer) (01-08-2020)

Worked well for WB

  • Applications: Western Blot,
  • Primary Antibody Dilution: 5000
  • Cell Tissue Type: HEKs
FH

Benjamin (Verified Customer) (01-07-2020)

Easily detects recombinant GFP protein via western blot with very little background.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2500 to 1:5000
  • Cell Tissue Type: Recombinant GFP protein
FH

Jing (Verified Customer) (01-03-2020)

Used this to detect tranduce efficiency of the AAV-GFP virus. with 5% non-fat milk, there are two bands around 20-30kd, not sure which one is correct. And the antibody is relative weak, has to use 1:500 dilution.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: mouse heart
FH

Shan (Verified Customer) (12-25-2019)

The GFP antibody showed great sensitivity for WB and it was easily detecable. But it was insteresting that when the SDS PAGE gel separating gel concentration reeached to 12%, you can see two bands at ~30kDa and ~20kDa.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: 293T
GFP tag Antibody Western Blot, validation (1:2500 dilution) in 293T (Cat no:66002-1-Ig)
FH

Jason (Verified Customer) (11-04-2019)

This is a good antibody for detecting GFP tag on Western blot, using 1:1000 dilution. The price is unbeatable, worth each penny.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: GFP-tagged protein expressed in human cell lines
FH

Hend (Verified Customer) (10-14-2019)

antibody used in western blot against egfp labelled protein and clear band was detected.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1 to 4000