CoraLite®594-conjugated GFP tag Monoclonal antibody

GFP tag Monoclonal Antibody for FC, IF, WB

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein

Applications

WB, IF, FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E10H7

Cat no : CL594-66002

Synonyms



Tested Applications

Positive WB detected inRecombinant protein
Positive IF detected inTransfected HEK-293 cells
Positive FC detected inTransfected HEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66002-1-Ig

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66002 targets GFP tag in WB, IF, FC applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GFP tag fusion protein Ag2128
Full Name GFP tag
Calculated molecular weight 26 kDa
GenBank accession numberU73901
Gene symbol
Gene ID (NCBI)
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Green fluorescence protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) derived from the jellyfish Aequorea Victoria which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm). GFP, when exposed to light in the blue to ultraviolet spectrum, will show a bright green fluorescent light, making it a very useful tool in research.

What is the molecular weight of GFP?

26.9 kDa

How does GFP work?

GFP was first isolated from the jellyfish Aequorea Victoria, a source of bioluminescence, in the 1960s and in 2008 the Nobel Prize in Chemistry was awarded "for the discovery and development of the green fluorescent protein, GFP" to Osamu Shimomura and colleagues, who recognized its potential in research (PMID: 13911999). A short amino acid sequence within the protein acts as the chromophore, which absorbs UV light at 395 nm and emits green light at 509 nm. 

Why is GFP a useful reporter?

When GFP was sequenced in 1992 (PMID: 1347277) it allowed scientists to express it in other organisms using transgenic techniques. It does not require cofactors to work, is non-toxic to live cells, and is relatively small, making it ideal as a "tag" for other proteins, identifiable by shining a UV light and observing the green fluorescence. The tertiary folded structure of GFP forms a chromophore at the center of a barrel shape, which protects the fluorescence-emitting amino acid chain from solvents, meaning it can function in many environments (PMID 9759496).

What are the applications for GFP?

When expressed attached to another protein, GFP can be used as a reporter gene to measure expression levels or can easily be used in fluorescence microscopy. It has been used to highlight proteins in a variety of model organisms, including bacteria, zebrafish, and mice.



Protocols

Product Specific Protocols
WB protocol for CL594 GFP tag antibody CL594-66002Download protocol
IF protocol for CL594 GFP tag antibody CL594-66002Download protocol
FC protocol for CL594 GFP tag antibody CL594-66002Download protocol
Standard Protocols
Click here to view our Standard Protocols