CoraLite® Plus 488-conjugated GFP tag Monoclonal antibody

GFP tag Monoclonal Antibody for WB, IF

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein

Applications

WB, IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E10H7

Cat no : CL488-66002

Synonyms

CFP, eGFP, eYFP, GFP, GFP-tag, green, green fluorescence protein, YFP



Tested Applications

Positive WB detected inRecombinant protein
Positive IF detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66002 targets GFP tag in WB, IF applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GFP tag fusion protein Ag2128
Full Name GFP tag
Calculated Molecular Weight 26 kDa
GenBank Accession NumberU73901
Gene Symbol
Gene ID (NCBI)
RRIDAB_2919276
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP. This antibody is labeled with CoraLite Plus 488.


Protocols

Product Specific Protocols
WB protocol for CL Plus 488 GFP tag antibody CL488-66002Download protocol
IF protocol for CL Plus 488 GFP tag antibody CL488-66002Download protocol
Standard Protocols
Click here to view our Standard Protocols