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AuthentiKine™ ELISA kits are Proteintech’s premium line of ELISA kits for measuring minute quantities of human serum factors:
To achieve superior sensitivity for human samples, these kits are built from the ground up using HumanKine® human-cell expressed cytokines and growth factors, in contrast to the industry standard of E. coli-derived proteins.
AuthentiKine™ ELISA kit development starts with production of HumanKine® immunogens, which are human proteins with native glycosylation and processing. Due to the highest quality immunogens in the industry, the capture and detection antibodies have the highest affinity for human proteins. To best mimic the human sample analyte for optimizing the antibody parameters, HumanKine® proteins are used as the standard. This means that the antibody manufacturing and conditions for optimizing are built around using human proteins as they are in nature, resulting in unparalleled sensitivity and accuracy.
|Minimum Detectable Dose (pg/mL)|
|Target||Fold better sensitivity||Proteintech||Leading Competitor|
|Human Cystatin C||10X||10.5||102|
Comparison of Proteintech’s AuthentiKine™ ELISA kits’ sensitivity versus top competitor’s kits determined by adding two standard deviations to the concentration corresponding to the mean optical density of 20 zero standard replicates.
|ELISA Kit||Catalog number||Sample Type||Sensitivity (pg/mL)|
|Human IL-6 ELISA kit||KE00139||serum, plasma, cell culture supernatant||1.2|
|Human IFN-gamma ELISA kit||KE00146||serum, plasma, cell culture supernatant||0.4|
|Human Cystatin C ELISA kit||KE00150||serum, plasma, cell culture supernatant, urine, saliva and human milk||10.5|
|Human TNF-alpha ELISA kit||KE00154||serum, plasma, cell culture supernatant||8.6|
|Human HGF ELISA kit||KE00168||serum, plasma, cell culture supernatant||260|
|Human IL-10 ELISA kit||KE00170||serum, plasma, cell culture supernatant||2.3|
|Human beta-NGF ELISA Kit||KE00174||Serum, plasma, Cell culture supernatants||0.1|
|Human IL-27 ELISA Kit||KE00182||Serum, plasma||0.1|
|Human M-CSF ELISA Kit||KE00184||Serum, Plasma, Cell culture supernatants, Urine, Saliva||1.1|