AuthentiKine™ Human IL-6 ELISA Kit

Sensitivity

1.2 pg/mL

Range

7.8-500 pg/mL

Reactivity

Human

Cat no : KE00139

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6



Product Information

KE00139 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL6 ELISA kit is to be used to detect and quantify protein levels of endogenous IL6. The assay recognizes human IL6. An antibody specific for IL6 has been pre-coated onto the microwells. The IL6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL6 is added to detect the captured IL6 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name AuthentiKine™ Human IL-6 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 1.2 pg/mL
Range 7.8-500 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3569

Recovery

Sample TypeAverageRange
Human serum89%81%-103%
Cell culture supernatants98%86%-106%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120270.79.43.5
22064.53.65.6
32014.90.63.7

InterAssay

Samplenmean ( pg/mL)SDCV%
124288.416.55.7
22463.32.03.2
32414.80.53.5

Background Information

Interleukin-6 (IL6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL6 Plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6

Publications

SpeciesSample TypeTitle
human,mousecell culture supernatants

BMC Med

HBeAg mediates inflammatory functions of macrophages by TLR2 contributing to hepatic fibrosis.

Authors - Xiaoyu Xie
humancell culture supernatants

Oncogenesis

Erlotinib overcomes paclitaxel-resistant cancer stem cells by blocking the EGFR-CREB/GRβ-IL-6 axis in MUC1-positive cervical cancer.

Authors - Yaping Lv
humancell culture supernatants

Nanotoxicology

Role of inflammation in the malignant transformation of pleural mesothelial cells induced by multi-walled carbon nanotubes.

Authors - Xiaopei Huang
humanserum

Emerg Microbes Infect

Serological investigation of asymptomatic cases of SARS-CoV-2 infection reveals weak and declining antibody responses.

Authors - Yong Yang
humanserum

Front Cell Dev Biol

miR-7-5p Affects Brain Edema After Intracerebral Hemorrhage and Its Possible Mechanism.

Authors - Xiqian Chen
humanserum

Front Immunol

Circulating Type I Interferon Levels in the Early Phase of COVID-19 Are Associated With the Development of Respiratory Failure.

Authors - Kentaro Nagaoka