AuthentiKine™ Human IL-6 ELISA Kit

Sensitivity

1.2 pg/mL

Range

7.8-500 pg/mL

Reactivity

Human

Cat no : KE00139

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6



Product Information

KE00139 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL6 ELISA kit is to be used to detect and quantify protein levels of endogenous IL6. The assay recognizes human IL6. An antibody specific for IL6 has been pre-coated onto the microwells. The IL6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL6 is added to detect the captured IL6 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name AuthentiKine™ Human IL-6 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 1.2 pg/mL
Range 7.8-500 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human serum89%81%-103%
Cell culture supernatants98%86%-106%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120270.79.43.5
22064.53.65.6
32014.90.63.7

InterAssay

Samplenmean ( pg/mL)SDCV%
124288.416.55.7
22463.32.03.2
32414.80.53.5

Background Information

Interleukin-6 (IL6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL6 Plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6

Publications

SpeciesSample TypeTitle
humancell culture supernatants

Front Oncol

Senolytics (DQ) Mitigates Radiation Ulcers by Removing Senescent Cells.

Authors - Huilan Wang
humancell culture supernatants

Oxid Med Cell Longev

Catalpol-Induced AMPK Activation Alleviates Cisplatin-Induced Nephrotoxicity through the Mitochondrial-Dependent Pathway without Compromising Its Anticancer Properties.

Authors - Jiangnan Zhang
humanserum

BMC Emerg Med

Endothelial glycocalyx degradation is associated with early organ impairment in polytrauma patients.

Authors - Feng Qi
humancell culture supernatants

Exp Ther Med

Quercetin suppresses inflammatory cytokine production in rheumatoid arthritis fibroblast-like synoviocytes

Authors - HAI‑TAO SUN
humanserum

Hemodial Int

Biomarkers upon discontinuation of renal replacement therapy predict 60-day survival and renal recovery in critically ill patients with acute kidney injury.

Authors - Tingting Yang
humancell culture supernatants

Ann Hepatol

MicroRNA-124a contributes to glucocorticoid resistance in acute-on-chronic liver failure by negatively regulating glucocorticoid receptor alpha.

Authors - Xin Wang