gp130/IL6ST Polyclonal antibody

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  WB analysis using 32186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32186-1-AP (gp130/IL6ST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

gp130/IL6ST Polyclonal Antibody for WB, ELISA

Cat No. 32186-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

IL-6R-beta, IL-6RB, IL-6R subunit beta, IL-6 receptor subunit beta, gp130

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Tested Applications

• Positive WB detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32186-1-AP targets gp130/IL6ST in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 6 signal transducer
• Calculated Molecular Weight: 102kDa, 917aa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: NM_010560.3
• Gene Symbol: Il6st
• Gene ID (NCBI): 16195
• RRID: AB_3670217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q00560
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GP130, also known as IL6ST, is a transmembrane protein that serves as a shared signal-transducing subunit for a variety of cytokines(PMID: 39414364), including interleukin-6 (IL-6), IL-11, IL-27, leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine (CLC)/CLC factor 1 (CLCF-1), and neuropoietin (PMID: 24099136). This protein is highly conserved among vertebrates and plays a crucial role in mediating interactions between a wide range of immune and non-immune cells. GP130 was originally discovered in 1978 and was successfully cloned and expressed in 1990, leading to a deeper understanding of cytokine signaling pathways (PMID: 308233).