• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Recombinant Human PCSK9 protein (Myc Tag, His Tag)

Species

Human

Purity

>95 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

EC50: 3-12 ng/mL

Cat no : Eg0199

Validation Data Gallery

  • Purity of Recombinant Human PCSK9 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human PCSK9 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human PCSK9 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Biotinylated Human LDL R (GST tag) with a linear range of 3-12 ng/mL.

    Immobilized Human PCSK9 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Biotinylated Human LDL R (GST tag) with a linear range of 3-12 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human PCSK9 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Biotinylated Human LDL R (GST tag) with a linear range of 3-12 ng/mL.
Expression HEK293-derived Human PCSK9 protein Gln31-Gln692 (Accession# Q8NBP7) with a Myc tag and a His tag at the C-terminus.
GeneID 255738
Accession Q8NBP7
PredictedSize 13.8 kDa (Propeptide) and 62.3 kDa (mature chain)
SDS-PAGE 17 kDa (Propeptide) and 65-75 kDa (mature chain), reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a crucial protein governing the circulating levels of low density lipoprotein-cholesterol (LDL-C), by virtue of its pivotal role in the degradation of the LDL receptor (LDLR). PCSK9 is expressed in the kidney and lung. It is synthesized as a 72 kDa immature precursor that undergoes autocatalytic cleavage in the endoplasmic reticulum to generate a 63 kDa mature protein. The cleaved N-terminal fragment remains associated with the mature protein and is necessary for its secretion, allowing it to circulate in the blood. The ability of PCSK9 to regulate a diverse group of cell-surface proteins hinted that it might also be able to influence additional membrane proteins that are important in anti-tumour immune responses. Targeting PCSK9 to treat cancer is also attractive because two neutralizing antibodies against it, evolocumab and alirocumab, have already been approved for human clinical use to lower cholesterol levels.

References:

1. Blanchard V, et al. (2019) Pathology. Feb;51(2):177-183.
2. Sharotri V, et al. (2012) J Biol Chem. Jun 1;287(23):19266-74
3. Canuel M, et al. (2013) PLoS One. May 13;8(5):e64145.

Publications

SpeciesTitle

Mol Ther

Engineered extracellular vesicles as nanosponges for lysosomal degradation of PCSK9

Authors - Chen Wang
View Article